Organic Acids

Synonyms

    Applications:

    HPLC Analysis of Calcium Ions in Maple Syrup

    2012-07-10

     

    Application Notes: maple syrup primarily consists of sucrose and other sugars, but also has small amounts of amino acids (arginine, threonine, proline), organic acids (malic and fumaric), vitamins (niacin and riboflavin) and traces of minerals (Na, K, Ca, Mn, Mg, Zn ions). One analysis performed on maple syrup is calcium ion determination. A Primesep 100 column was used in cation-exchange mode to isolate calcium ion from other components of the maple syrup. This method can be used for quantification of calcium ion by ELSD/CAD. Limit of detection depends on the type of ELSD/CAD detector used but usually reaches 20 ppm

    Application Columns: Primesep 100
    Application compounds: calcium ions, Maple Syrup
    Detection technique: LC/MS, ELSD/CAD

    Condition

    Column Primesep 100, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer TFA
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Ions, Hydrophilic, Ionizable
    Analyzing Compounds Maple Syrup,  Calcium

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Calcium
    Organic Acids

    HPLC Separation of Organic Acids

    2012-07-10


    Primesep D mixed-mode column separates organic acids such as succinic acid, malic acid, MPS and butanesulfonate by a mixture of anion exchange and reversed phase mechanisms. Retention times can be changed by adjusting the percentage of acetonitrile in the mobile phase. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium formate as a buffer, making the method MS-compatible. Can also use UV detection at 250 nm.

    Condition

    Column Primesep D, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds Sodium, Succinic Acid, Malic Acid, MPS, Butanesulfonate

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep D

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Butanesulfonate
    Malic Acid
    Organic Acids
    Sodium
    Succinic Acid

    Application Detection:
    UV Detection
    ELSD/MS Detection

    HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column

    2011-07-14

    Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).

    Condition

    Column Primesep S2, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 85/15%
    Buffer AmAc pH 5.0 15 mM
    Flow Rate 1.0 ml/min
    Detection ELSD, 50C UV 250 nm

     

    Description

    Class of Compounds
    Nucleosides,  Hydrophilic, Ionizable
    Analyzing Compounds Toluenesulfonic acid, Naphthalenedisulfonic acid, 3.5 DHBA, Ascorbic acid,

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep S2

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    1,5-Naphthalenedisulfonic Acid
    3,5-Dihydroxybenzoic Acid
    Ascorbic Acid
    Organic Acids
    p-Toluenesulfonic Acid (PTSA)

    Application Detection:
    UV Detection

    Separation of Maleic and Succinic Acid on Primesep B2 column

    2011-07-08

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    Organic and inorganic acids can be retained and separated on mixed-mode columns based on weak reversed-phase and weak/medium anion-exchange mechanisms. Amount of ACN, buffer concentration and buffer pH will affect retention time of organic and inorganic acids. Acids can be monitored by low UV, ELSD or LC/MS. Presence of ions is required to facilitate ion-exchange mechanism. Method can be used as a general approach for analysis of acidic hydrophilic and acidic hydrophobic compounds. Carboxylic acids along with inorganic acid can be retained and separated without ion-pairing reagent.

    Condition

    Column Primesep B2, 4.6×50 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 10/90%
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection ELSD, UV 230nm

    Description

    Class of Compounds Acid, Hydrophilic, Ionizable
    Analyzing Compounds Succinic Acid, Maleic Acid
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep B2

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Maleic Acid
    Organic Acids
    Succinic Acid

    Application Detection:
    UV Detection
    ELSD/MS Detection

    HPLC Separation of Pyridinecarboxylic Acids

    2011-03-27


    Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Isonicotinic Acid
    Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
    Organic Acids
    Picolinic Acid
    Pyridinedicarboxylic Acid

    Application Detection:
    UV Detection

    HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids on Newcrom BH Column

    2010-12-06

    Separation type: Liquid Chromatography Mixed-mode




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    Condition

    Column Newcrom BH, 3.2×50 mm, 3 µm, 100A
    Mobile Phase MeCN/H2O – 40/60%
    Buffer H3PO4 – 3%
    Flow Rate 0.5 ml/min
    Detection UV 275nm


    Description

    Class of Compounds Acid
    Analyzing Compounds Benzenesulfonic, p-Toluenesulfonic Acids

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Newcrom BH

    The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

    Select options
    Application Analytes:
    Benzenesulfonic Acid
    Organic Acids
    p-Toluenesulfonic Acid (PTSA)

    Application Detection:
    UV Detection

    HPLC Separation of Inorganic Anions

    2010-11-21


    Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.

    Condition

    Column Primesep B4, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Ions, Hydrophilic, Ionizable
    Analyzing Compounds Sodium, Phosphate, Chloride,  Nitrate, Sulfate, Iodide, Perchlorate, Trifluoracetic

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep B4

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Hydrochloric Acid
    Methanesulfonic Acid
    Nitrate
    Nitric Acid
    Organic Acids
    Perchloric Acid
    TFA (Trifluoroacetic Acid)

    Application Detection:
    ELSD/MS Detection

    HPLC Separation of Succinic and Methylmalonic Acids

    2010-11-12


    Organic and inorganic acids can be separated by reversed-phase anion-exchange Primesep D column. Succinic and methylmalonic acids were separated based on their hydrophobic and ionic properties. Method can be used for analysis of other acids by HPLC.

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep D

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Methylmalonic Acid
    Organic Acids
    Succinic Acid

    Application Detection:
    ELSD/MS Detection

    HPLC Separation of Lactic Acid and Its Oligomers

    2010-07-15


    Lactic acid (milk acid) is natural organic acid that plays an important role in biochemical processes. It has carboxylic and hydroxy-groups which upon standing can react with each other and form dimers, trimers and higher oligomers. Lactic acid is a polar molecule that produces no retention on reversed-phase column. Primesep B2 mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Oligomers of lactic acid can be converted to lactic acid by hydrolysis. Primesep B2 column can be used for the analysis of lactic acid alone and lactic acid in the mixture of oligomers. Lactates can be monitored by low UV, ELSD or LC/MS. In case of ELSD, CAD or LC/MS, phosphoric acid in the mobile phase needs to be replaced with buffer compatible with these detection techniques (ammonium formate or TFA).

    Condition

    Column Primesep B2, 3.2×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer H3PO4 – 0.1%
    Flow Rate 0.5 ml/min
    Detection UV 210 nm

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds Lactic Acid

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep B2

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Lactic Acid
    Lactic Acid Oligomers
    Organic Acids

    Application Detection:
    UV Detection

    HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography

    2009-07-16

    The majority of drugs in the pharmaceutical industry are administered in salt form. The presence of two counter-ions very often necessitates the use of two methods. The nature of these counterparts in drugs can be an inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Furthermore, the properties of the molecules will result in a differing stoichiometry. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by the reversed-phase mechanism, and counter-ions are retained by the reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by the anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing the amount of acetonitrile and the amount of ions in the mobile phase. The detection technique depends on the properties of the counter-ions. In case of low or no UV activity, ELSD can be employed if the counter-ion forms a non-volatile salt with the mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. The presence of two mechanisms of retention allows control over retention times of drug and counter-ion independently, and even allows a change of order of elution when necessary.

    Condition

    Column Primesep D , 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection ELSD, UV 270

    Description

    Class of Compounds Ions, Hydrophilic, Hydrophobic, Base, Acids, Ionizable
    Analyzing Compounds Sodium Chloride, Sodium chloride, Sodium Chlorate, Sodium bromide, Sodium bromate, Perchloric Acid, Maleic Acid, Fumaric Acid, Tartaric Acid, Succinic Acid, Phosphoric Acid, Citric acid, Benzosulfonic acid,  Dextromethorphan, Verapamil, Trimipramine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep D

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Benzenesulfonic Acid
    Bromide
    Chlorate
    Chloride
    Citric Acid
    Dextromethorphan
    Fumaric Acid
    Maleic Acid
    Organic Acids
    Perchlorate
    Phosphoric Acid
    Pyrilamine
    Succinic Acid
    Tartaric Acid
    Verapamil
    p-Toluenesulfonic Acid (PTSA)

    Application Detection:
    UV Detection
    ELSD/MS Detection

    HILIC Separation of Aromatic Acids

    2008-08-22

    Obelisc N column are used for separation of weak and strong organic acids in mixed-mode HILIC. Benzoic and naphthalenesulfonic acids are retained based on polar interaction mode and anion-exchange mode. Order of elution and retention pattern can be changed by modifying mobile phase. PH of the mobile phase changes ionization state of stationary phase and analytes. Fast quantitation method for benzoic and naphthalenesulfonic acid can be developed using UV, ELSD or LC/MS detection. HPLC Method can be used for mixture of organic and inorganic strong and weak acids.

    Condition

    Column Obelisc N, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmAc
    Flow Rate 1.0 ml/min
    Detection UV, 270 nm

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds  Benzoic acid, Naphthalenesulfonic Acid

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Obelisc N

    SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Benzoic Acid
    Naphthalenesulfonic Acid
    Organic Acids

    Application Detection:
    UV Detection

    HILIC Separation of Carboxylic Acids

    2008-08-22

    Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Obelisc N

    SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Fumaric Acid
    Hydroxybenzoic Acid
    Malic Acid
    Mandelic Acid
    Methylmalonic Acid
    Organic Acids
    Succinic Acid
    Tartaric Acid

    Application Detection:
    ELSD/MS Detection

    HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column

    2007-12-06

    Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.

    Condition

    Column Primesep  N , 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmAc
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Acid, Vitamin B₆, Hydrophobic, Ionizable
    Analyzing Compounds Ascorbic acid (Vitamin C), Methylmalonic acid,  Succinic acid
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep N

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Ascorbic Acid
    Methylmalonic Acid
    Organic Acids
    Succinic Acid

    Application Detection:
    ELSD/MS Detection

    HPLC Separation of Organics Acids

    2006-11-21

    Primesep D separates organic acids such as fumaric, benzoic, phthalic, naphthoic, and maleic acids by a mixture of anion exchange and reversed phase. Retention times and elution order can be changed by adjusting the percentage of acetonitrile in the mobile. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.

    Condition

    Column Primesep D, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm
    Flow Rate 1.0 ml/min
    Detection UV 250 nm

     

    Description

    Class of Compounds
    Acid, Hydrophilic, Ionizable
    Analyzing Compounds Fumaric Acid, Benzoic Acid, Phthalic Acid, Maleic Acid, Naphtoic Acid

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep D

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Benzoic Acid
    Fumaric Acid
    Maleic Acid
    Naphthoic Acid
    Organic Acids
    Phthalic Acid

    Application Detection:
    UV Detection

    Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds

    2006-10-14

    Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection.

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer TFA – 0.2
    Flow Rate 1.0 ml/min
    Detection UV, 210 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Tyrosine, phenylalanine, Benzoic acid, mandelic acid, Benzylamine, Pyridine, Benzonitrile, Toluene

     

    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Benzoic Acid
    Benzonitrile
    Benzylamine
    Mandelic Acid
    Organic Acids
    Phenylalanine
    Pyridine
    Toluene
    Tyrosine

    Application Detection:
    UV Detection