Cannabis Analysis by HPLC


Cannsep A chromatogramCannsep SB 10 chromatogram

Cannsep C chromatogram

Simplified Cannabis Analysis


  • Easy sample preparation
  • Method developed by people who understand chromatography
  • No chemical experience is required for instrument user
  • Step by step instructions
  • User friendly software


Analysis by HPLC is the most reliable and accurate method of measuring active components in Cannabis products. SIELC has developed special line of columns – Cannsep, which allows to resolve most of the compounds attributed to cannabis physiological properties. Three columns, Cannsep A, Cannsep B, and Cannsep C resolved all the cannabinoids providing different and significantly orthogonal selectivity.

The liquid chromatography does not require high temperature which is the case in gas chromatography (GC) analysis.  No thermo-degradation of compounds occurs during the test.

In addition to the Cannsep columns, SIELC provides the Alltesta Analyzer, a device that allows for easy and efficient analysis of cannabis products. The Alltesta Analyzer is a user-friendly tool that automates the HPLC testing process and provides accurate and reliable results in a matter of minutes. With the Alltesta Analyzer, you can perform quantitative analysis of components in cannabis products with minimal training and setup time.

Columns are available in all standard dimensions and with particles 5 µm for regular HPLC and 3 µm for UPLC (Ultra-high Pressure Liquid Chromatography). If you contract out this test you will pay over $100 per sample. The Cannsep columns are the only consumable that you will ever need to be able to perform such analysis in your own shop or home. One column can be used for a multitude of tests.

The incomplete list of compounds that can be analyzed by this HPLC method includes:

  1. Cannabidivarin (CBDV)
  2. Tetrahydrocannabivarin (THCV)
  3. Cannabidiol (CBD)
  4. Cannabidiolic Acid (CBDA)
  5. Cannabigerol (CBG)
  6. Cannabinol (CBN)
  7. Delta-8-Tetrahydrocannabinol, Δ-8-THC
  8. Delta-9-Tetrahydrocannabinol, Δ-9-THC
  9. Cannabichromene (CBC)
  10. Tetrahydrocannabinolic acid (THCA)


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Cannabis Analysis by HPLC Products