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Applications:
- Proteomics
- Peptide/Protein Analysis
- Peptide/Protein Purification
Features & Benefits:
- 2-D HPLC with a Single Column
- Ionic Interactions
- Reversed-Phase
- Separate peptides and proteins that differ only by a single amino acid
- Enhanced selectivity for closely related peptides and proteins.
- Increased peak capacity compared to reversed-phase and ion exchange.
- Increased LC-MS sensitivity using formic acid and ammonium acetate.
- Completely Scalable from capillary to prep
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Promix™ Technology
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Independent control of acid/buffer concentration and organic modifier
offers almost limitless control of retention and selectivity!
1. ARG-VAL-TYR-VAL-HIS-PRO-ILE
2. ARG-VAL-TYR-VAL-HIS-PRO-PHE
3. ALA-PRO-GLY-ASP-ARG-ILE-TYR-VAL-HIS-PRO-PHE
4. ASP-ARG-VAL-TYR-VAL-HIS-PRO-PHE-HIS LEU
5. ASP-ARG-VAL-TYR-ILE-HIS-PRO-PHE-HIS-LEU |
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Upper Column: Promix TM MP, 4.6 x 250 mm 300 Å Mobile phase: MeCN – gradient 15-35 % in 15 min Gradient TFA – 0.4-0.05% in 15 min;
Lower Column: Promix TM MP, 4.6 x 150 mm 300 Å Mobile phase: MeCN – gradient 0-40 % in 30 min
Gradient TFA – 0.4-0.05% in 30 min;
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1. Ribonuclease A (mw 13,500)
2. Insulin (mw 5,700)
3. Lysozyme (mw 14,300)
4. B-Lactalbumin (mw 14,200)
5. Carbonic Anhydrase (mw 29,000) |
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