Legacy columns
SIELC manufactures Legacy L1, Legacy L3, Legacy L7, Legacy L8, Legacy L9, Legacy L10, Legacy L11, Legacy L12, Legacy L13, Legacy L14, Legacy L16, Legacy L18, Legacy L20, Legacy L26, Legacy L28, Legacy L44, Legacy L78 and Legacy L85 columns.
As the HPLC world adapts to scientific advancements in separation technologies, old column technologies can easily become obsolete enough to warrant chromatography companies to stop producing these columns. Sometimes, companies will shut down only that production line, and other times, companies will shutter completely as they are unable to evolve quickly enough with the ever-changing landscape.
Consumers that once depended on these out-of-production columns are now stuck – they can either overhaul entire methods and systems, or they can abandon their projects altogether. With SIELC’s Legacy column series, consumers don’t have to do either.
SIELC’s Legacy column series is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. ple replacement. For example, L1 is one of the most popular designations, referring to columns with octadecyl silane (C18) chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter. An L1 column from one manufacturer can be swapped for another L1 column from a different manufacturer with little to no modification of the method. However, not all L1 columns are exactly the same, so relative compound retention and peak shape can be different from one brand’s column to another.
For companies that have developed integral methods based on USP columns that are no longer in production, the Legacy column series offers them a simple and easy way to find a compatible replacement.
If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here
If you would like to purchase these columns please contact us at sales@sielc.com
| SIELC Column | USP Specification |
| Legacy L1 | Octadecyl silane chemically bonded to porous silica or ceramic particles (3 to 10µm) |
| Legacy L3 | Porous silica microparticles 1.5 to 10µm in diameter |
| Legacy L7 | Octyl silane (C8) chemically bonded to porous silica particle- 3 to 10µm in diameter |
| Legacy L8 | An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3-10µm diameter |
| Legacy L9 | Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter. |
| Legacy L10 | Nitrile groups chemically bonded to porous silica particles 3 to 10µm in diameter |
| Legacy L11 | Phenyl groups chemically bonded to porous silica particles 3 to 10µm in diameter |
| Legacy L12 | A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 3 to 50 µm in diameter |
| Legacy L13 | Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter |
| Legacy L14 | Silica gel, 5 to 10µm in diameter, having a chemically bonded, strongly basic quaternary ammonium anion exchanger (SAX) coating. |
| Legacy L16 | Dimethylsilane (C2) chemically bonded to totally porous silica particles- 5 to 10µm. |
| Legacy L18 | Amino and cyano groups chemically bonded to porous silica particles, 5 to 10µm in diameter |
| Legacy L20 | Dihydroxypropane (diol) groups chemically bonded to porous silica or hybrid particles, 3 to 10µm in diameter. |
| Legacy L26 | Butyl silane (C4) chemically bonded to porous silica particle- 3 to 10µm. |
| Legacy L28 | A multifunctional support which consists of a high purity, 100A, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to conventional reversed-phase C8 functionality. |
| Legacy L44 | A multifunctional support, which consists of high purity, 100A, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a convention reversed-phase C8 functionality |
| Legacy L78 | A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, or tertiary amino groups) functional groups chemically bonded to porous or non-porous or ceramic micro-particles, 3 to 50µm in diameter or a monolithic rod |
| Legacy L85 | A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 3 to 50µm in diameter |
If you would like to purchase these columns please contact us at sales@sielc.com
Development of new Methods
While USP has produced a list of validated methods, many people alter USP methods to better suit their needs. Many of the methods listed in USP are outdated because they were developed on older instruments with outdated column technologies. Therefore, they might need to be modified to better suit new instrumentation and columns. Altering the validated USP methods can save times and improved results.
Legacy Products
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Legacy L1
The Legacy L1 column is a silica-based, single-mode reverse-phase C18 column that meets the USP qualifications for the L1 designation. According to the USP definition for an L1 stationary phase, it requires octadecyl silane chemically bonded to porous silica or ceramic particles ranging in size from 1.5 to 10 µm. This stationary phase retains compounds based on their hydrophobic interactions with the ligands. If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here. Select options -
Legacy L16
The Legacy L16 column is a silica-based, single-mode reverse-phase C12 column that meets the USP qualifications for the L16 designation. The USP definition for an L16 stationary phase requires dimethylsilane (C2) chemically bonded to totally porous silica particles (5 to 10 µm). Compounds are retained based on their hydrophobic interactions with the ligands. If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here. Select options -
Legacy L3
The Legacy L3 column is a single-mode, normal phase column that contains porous silica, without any ligands or additional chemistry attached to the surface. The Legacy L3 column meets the USP qualifications for the L3 designation, which is defined as a column packed with porous silica microparticles 1.5 to 10μm in diameter (with no additional surface chemistry). If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here. Select options -
Legacy L8
The Legacy L8 column is a single-mode, ion-exchange column with aminopropylsilane (AP) groups chemically bonded to totally porous silica gel micro-particles. The Legacy L8 column meets the UPS standard for the L8 designation, which states that the column consists of an essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3-10μm diameter. The AP groups allow for both cation-exclusion and hydrophilic interaction chromatography (HILIC). If you would like to learn more about SIELC's line of Legacy columns, you can download the brochure here. Select options
