Mixed-mode Stationary Phases
For decades, liquid chromatography stationary phase design has been dominated by the idea of elimination of multiple, or “unwanted” interactions that occur in mixed-mode separations. Reverse-phase chromatography is making the point of eliminating silanol interactions with amine-containing analytes by developing base-deactivated phases.
Within the same trend, adsorption interactions are considered undesirable as opposed to steric interactions in size-exclusion chromatography, and non-ionic interactions are generally viewed as complications in ion-exchange and ion-exclusion chromatography.
However, there are ways to benefit from multiple interactions on the stationary phase. SIELC Technologies introduces Primesep® HPLC columns, specifically designed for mixed-mode separations and capable of separating a tremendous range of compounds by different separation modes based only upon mobile phase selection. MORE
Currently, SIELC Technologies offers the following types of silica-based mixed-mode phases.
Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent.
|Strongest acidic phase
|Strong acidic phase
|Medium acidic phase
Choose the correct column by their functional group acidity to match the pKa value of your basic compounds. MORE
Columns with positively charged functional groups. Primesep B
Strong basic column for use with TFA, phosphoric or perchloric acid solutions in a mobile phase. Primesep B2
Weak basic column for ammonium acetate buffered solution. MORE
Column with unique unparalleled selectivity. Primesep C
Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions. MORE
Primesep 100Select options
Primesep 200Select options
Primesep 500Select options
Primesep ASelect options
Primesep ABSelect options
Primesep APSelect options
Primesep BSelect options
Primesep B2Select options
Primesep B4Select options
Primesep CSelect options
Primesep DSelect options
Primesep NSelect options
Primesep PSelect options
Primesep PBSelect options
Primesep SSelect options
Primesep S2 is a silica-based, acidic column with hydrophilic properties. It provides the following capabilities: - Separates basic compounds using a cation-exchange mechanism. - Separates basic and neutral polar compounds through a HILIC (Hydrophilic Interaction Liquid Chromatography) mechanism. The mobile phases can be tailored for various applications, such as LC-MS and preparative chromatography, and also optimized for low UV detection.Select options
Primesep SBSelect options