PRIMESEP COLUMN OPERATION AND STORAGE
Good column care requires little additional time and will extend the lifetime of your PRIMESEP HPLC columns. Column care for PRIMESEP brand of columns involves operating in a suitable mobile phase and storing PRIMESEP column in an appropriate solvent system.
Mobile Phases for Column Operation
To clean the column, use the mobile phase consisting of 60/40/0.3 ACN/H2O/H2SO4.
The column can also be run in reverse at a reduced flow rate 2-3 times.
If your sample matrix contains many compounds, especially multi-charged compounds like proteins, we recommend the use of a guard column and replace it often as soon as you notice degradation in performance, i.e. peak shape, shift in retention, etc.
If you run a high-throughput operation, we recommend the use of FlipLC, which eliminates the use of constantly changing guard columns.
Table 1. Operational pH ranges for SIELC Columns
|Column Packing||pH Limits/Recommended Operating Range||Short Term Storage||Long Term Storage||Alcohol in mobile phase|
|Primesep A, Primesep 100, Primesep 200, Primesep C, Primesep P, Primesep AB, Primesep B2, Obelisc R, Promix SP, Promix AP||1.5-7.0 / 2.0-6.5||Buffered or acidified mobile phases within recommended pH range||ACN / water / formic or acetic
ACN / water / acetic acid
|Primesep B, Primesep D, Primesep B4, Primesep SB, Primesep N, Primesep PB, Primesep S, Promix MP, Obelisc N||1.5-5.0 / 2.0-4.5||Mobile phases within recommended pH range||ACN / water / formic acid or acetic
|PEI||1.5-5.0||Mobile phase||Mobile phase||Allowed|
|SHARC||No water||Mobile phase||Mobile phase||Allowed|
|Newcrom||1.5-7||Mobile phase||ACN / water / formic acid or acetic
Preform the column performance test using conditions described in the Certificate of Analysis and compare with the result in the report. Due to variations in particular LC systems you may observe slightly different results.