The Legacy L1 column is a silica-based, single-mode reverse-phase C18 column that meets the USP qualifications for the L1 designation. According to the USP definition for an L1 stationary phase, it requires octadecyl silane chemically bonded to porous silica or ceramic particles ranging in size from 1.5 to 10 µm. This stationary phase retains compounds based on their hydrophobic interactions with the ligands.

If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.

Below we have shared a select sample of methods showcasing the retention capabilities of the Legacy L1 column.

Prednisolone is a synthetic corticosteroid that is used to treat many chronic ailments, such as colitis, multiple sclerosis, and arthritis. Figure 1 shows the retention of Prednisolone on a Legacy L1 column with a mobile phase (MP) of 30% Acetonitrile (MeCN) and 70% water.

Epinephrine, or adrenaline, is a naturally-occurring hormone and neurotransmitter. In the pharmaceutical industry, it is used to treat severe allergic reactions, heart attacks, and asthma. Figure 2 shows the retention of Epinephrine on a Legacy L1 column with a mobile phase primarily consisting of methanol (MeOH) and water, with NaH2PO4, Sodium 1-Octanesulfonate, and EDTA as buffers.

Ketoprofen is a nonsteroidal anti-inflammatory drug (NSAID) used frequently to help treat arthritis-related pain and gum inflammation caused by severe toothaches. Figure 3 shows retention of Ketoprofen on a Legacy L1 column with a mobile phase consisting of water and acetonitrile and a high concentration of monosodium phosphate (MSP, NaH₂PO₄).

Amoxicillin is a common antibacterial drug derived from penicillin. Figure 4 shows the retention of Amoxicillin on a Legacy L1 column with a mobile phase consisting of methanol and primarily water, with ammonium acetate as the buffer.