HPLC Analysis of Inosine on Legacy L1

 

 

 

Inosine is a nucleoside made of a hypoxanthine and a ribose ring. As a drug, Inosine is useful for the treatment of various autoimmune diseases. Legacy L1 was used to retain Inosine by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

Condition

Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 248 nm

 

Description

Class of Compounds
 Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds Inosine

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Inosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.