The Legacy L3 column is a single-mode, normal phase column that contains porous silica, without any ligands or additional chemistry attached to the surface. The Legacy L3 column meets the USP qualifications for the L3 designation, which is defined as a column packed with porous silica microparticles 1.5 to 10μm in diameter (with no additional surface chemistry).

If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.

Below, we have shared a select sample of methods showcasing the retention capabilities of the Legacy L3 column.

Sulfuric acid is a highly corrosive acid commonly used as a buffer in chromatography. Sulfamic acid is an intermediary between sulfuric acid and sulfamide. Figure 1 shows the separation and retention of sulfuric and sulfamic acid on a Legacy L3 column with a mobile phase consisting of MeCN, water, and formic acid. This separation also used a gradient to change the concentration of MeCN and formic acid over time.

Lecithins are a class of amphiphilic, fatty, yellow-brownish compounds that occur naturally in plant and animal tissues. Soy lecithin is lecithin derived from soy and is often used as an emulsifier in food production. Figure 31 shows how commercial-grade lecithin and soy lecithin can be retained on a Legacy L3 column with a mobile phase consisting of MeCN, water, and an ammonium formate buffer, using a gradient to control the buffer and MeCN concentrations over the course of the separation.