Universal Phases

Primesep 100 reverse and normal mode chromatograms - Benzoin, Deoxybenzoin, Benzil

Primesep® mixed-mode stationary phases

Primesep® mixed-mode stationary phases provide two functional groups for interaction with analytes.

One is a very hydrophobic alkyl chain; another is a very hydrophilic acidic residue (Primesep A, Primesep 100, Primesep 200) or a protonated base (Primesep B).

As these two functional groups substantially differ in polarity, multiple separation modes can be performed on the same column.

Neutral compounds can be resolved in reverse, normal, or polar organic mode. Charged molecules can be resolved in reverse, normal, polar organic, ion-exchange, or ion-exclusion modes. Ionizable molecules usually require a combination of two or more modes.

Selection of the separation mode is governed by the type of the mobile phase chosen for a particular separation.

Primesep 100 Ion exchange mode chromatogram Primesep 100 Ion exclusion mode chromatogram