High loading capacity observed for polar compounds on Primesep 100 column, which is important for preparative separation.
Isocratic method can be developed for separation of compounds with drastically different polarity. Primesep® columns are ideally suited for universal detectors such as ELSD and MS.
Often, compounds without chromophores have low hydrophobicity and retain poorly on reverse-phase columns. Ion-exchange columns show low selectivity to compounds with similar pKa value. In traditional ion-exchange chromatography non-volatile buffers are used. This greatly complicates application of ion-exchange chromatography with evaporative detectors such as MS and ELSD.
This example demonstrates the separation of amino acids on a Primesep 100 column by reverse-phase and ion-exchange mechanism.