Separation of a Complex Mixture containing very small peptides and very large peptides can be achieved using two different Promix columns. Very small peptides eluted in first 1.5 min on Promix TM MP column were collected and resolved using Promix SP column .

Albumin Digest Selectivity Affected by Buffer Concentration :
Complex mixtures such as protein digests have different profiles at different ionic strengths. Multiple gradients can be applied to complex samples:

  • organic gradient
  • buffer gradient
  • or both

Each mode provides a different selectivity and elution profile.