Mixed-Mode HPLC Separation

For decades, liquid chromatography stationary phase design has been dominated by the idea of elimination of multiple, or “unwanted”, interactions that occur in mixed-mode separations. Reverse-phase chromatography is making the point of eliminating silanol interactions with amine-containing analytes by developing base-deactivated phases.

Within the same trend, adsorption interactions are considered undesirable as opposed to steric interactions in size-exclusion chromatography, and non-ionic interactions are generally viewed as complications in ion-exchange and ion-exclusion chromatography. However, there are ways to benefit from multiple interactions on the stationary phase.

SIELC Technologies introduces a new line of silica based HPLC columns that exhibit superior retention of polar compounds.

  • Every Primesep® column has a unique stationary phase, combining the properties of hydrophobic and ion-exchange stationary phases.
  • Strong interaction between the two phases allows to control retention of ionizable and neutral compounds independently.
  • For complex separation, it offers control of retention by changing only the amount of organic modifier in your mobile phase and amount of acid.
  • The type of organic modifier and the type of acid will affect retention and selectivity of your separation. With almost infinite combination of mobile phase properties you can achieve optimum separation in any complex situation.
  • Whether you separate by reverse-phase mechanism, ion-exchange mechanism, ion exclusion mechanism, and size exclusion mechanism or a combination of those, Primesep® column alone can perform it for you.
  • No tailing associated with silica surface chemistry.
  • You can transfer your analytical method directly to MS or prep without modifications.
  • Two-dimensional mapping separation is available with a single Primesep® column.