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SHARC 1

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  • Applications
Column Diameter

0.5 mm, 1 mm, 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm

Column Length

10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard

Particle Size

3 µm, 5 µm

Pore Size

100 A

Brand

SIELC Technologies

SHARC™ 1 – The SHARC™ 1 column is the first of the SHARC™ series columns. SHARC™ columns are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC™ stands for Specific Hydrogen-bond Adsorption Resolution Chromatography.

Technology

SHARC™ 1 column operation conditions are unique. A mixture of acetonitrile (MeCN), a weak solvent, and  methanol (MeOH), a strong solvent, are used as the mobile phase. Pure MeCN  has very insignificant amount of hydrogen bonding with the SHARC stationary phase, while MeOH interacts strongly with SHARC™ stationary phase, which reduces the retention of analytes based on its capacity to the hydrogen bond. By changing the ratio of MeCN/MeOH, the optimum retention profile can be obtained for many types of molecules with high selectivity, peak shape, efficiency, and speed.

Hydrogen bonding interaction H bonding chromatography 

Surface interaction within the column with hydrogen, bonding capable analytes with acetonitrile as the mobile phase.

Surface interaction within the column with hydrogen, bonding capable analytes with methanol as the mobile phase.

 

 

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$392.70$7,553.70

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SHARC 1 Application Examples