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SHARC 1

  • Description
  • Purchase
  • Applications
Column Diameter

0.5 mm, 1 mm, 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm

Column Length

10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard

Particle Size

3 µm, 5 µm

Pore Size

100 A

Brand

SIELC Technologies

SHARC 1 – The SHARC 1 column is the inaugural member of the SHARC™ series. SHARC™ columns represent the first commercially available columns that primarily utilize separation based on hydrogen bonding. SHARC™ is an acronym for Specific Hydrogen-bond Adsorption Resolution Chromatography.

Technology

The operational conditions for the SHARC 1 column are unique. A blend of acetonitrile (MeCN), a weak solvent, and methanol (MeOH), a strong solvent, is used as the mobile phase. Pure MeCN forms very insignificant hydrogen bonds with the SHARC stationary phase, while MeOH interacts strongly, reducing the retention of analytes based on its capacity to form hydrogen bonds. By altering the MeCN/MeOH ratio, an optimal retention profile can be achieved for a wide range of molecules, offering high selectivity, peak shape, efficiency, and speed.

Hydrogen bonding interaction H bonding chromatography 

Surface interaction within the column with hydrogen, bonding capable analytes with acetonitrile as the mobile phase.

Surface interaction within the column with hydrogen, bonding capable analytes with methanol as the mobile phase.

 

 

SHARC™ columns represent the first commercially available columns that primarily utilize separation based on hydrogen bonding. SHARC™ is an acronym for Specific Hydrogen-bond Adsorption Resolution Chromatography.

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36-month financing with market rates is offered to companies, individuals, and educational institutions subject to credit approval. For more details please contact us at financing@sielc.com.

HPLC Determination of Succinic Anhydride and Succinic Acid on SHARC 1 Column

HPLC Determination of Piracetam on SHARC 1 Column

HPLC Determination of Imidazolidinyl urea on SHARC 1 Column

HPLC Determination of Allantoin on SHARC 1 Column

HPLC Separation of Acedoben and Inosine on SHARC 1 Column

HPLC Determination of Dicyandiamide on Sharc 1 Column

HPLC Separation of Pyrazine and Aminopyrazine on Sharc 1 Column

HPLC Separation of Mixture of DMF and DMSO

HPLC Analysis of Ethephon in Hydrogen-Bonding Mode

Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode

Paraquat and Diquat Separation in Non-aqueous Mobile Phase

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase

HPLC Dye Separation on SHARC1 Column

Methanol and Ethanol Concentration Effect on Alizarin Retention on SHARC1 Column

Retention of Taurine on SHARC Column

Analysis of Compounds of TCA Cycle on SHARC 1 Column

HPLC Separation of Nucleosides and Deoxynucleosides

HPLC Separation of Inosine and Deoxyinosine

HPLC Separation of the Nonionic Surfactant Triton-X-100

HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a SHARC 1 HPLC Column

HPLC Separation of Adenosine and Adenine Using the Hydrogen Bonding Method

HPLC Separation of Cytidine and Cytosine Using the Hydrogen Bonding Method

HPLC separation of Dihydroxybenzoic acid in Hydrogen-Bonding mode on SHARC 1 HPLC column

HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding

HPLC Separation of Phthalic Acids using Hydrogen Bonding