Retention of Taurine on SHARC Column

Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the SHARC 1 hydrogen-bonding column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.


Column Sharc 1, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Formic Acid - 0.1%, AmFm - 0.01%
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Taurine
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Application Analytes:

Application Detection:
ELSD/MS Detection