Retention of Taurine on SHARC Column

Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the SHARC 1 hydrogen-bonding column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.

Condition
Column Sharc 1, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Formic Acid – 0.1%, AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Taurine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Taurine
Zwitterion
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.