| CAS Number | 107-35-7 |
|---|---|
| Molecular Formula | C2H7NO3S |
| Molecular Weight | 125.140 |
| InChI Key | XOAAWQZATWQOTB-UHFFFAOYSA-N |
| LogP | -2.66 |
| Synonyms |
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Applications:
HPLC Method for Simultaneous Determination of Lactic Acid, Taurine, and Myo-Inositol on Primesep S2 Column
May 6, 2025
Separation type: Liquid Chromatography HILIC
HPLC Method for Analysis Lactic Acid, Taurine, Myo-Inositol on Primesep S2 Column by SIELC Technologies
Lactic Acid
Commonly used in food preservation, cosmetics, pharmaceuticals, and as a biomarker in physiological studies (e.g., exercise and metabolism).
Taurine
Widely present in energy drinks and supplements; important in cardiovascular function, development of the nervous system, and cellular protection.
Myo-Inositol
Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.
The mixture of lactic acid, taurine, and myo-inositol can be effectively retained and separated using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN), without the need for a buffer.
The mixture consists of lactic acid, an organic α-hydroxy acid; taurine, a sulfonic acid amino acid derivative; and myo-inositol, a polyol (sugar alcohol), representing diverse chemical classes with varying polarities and functional groups.
This method offers high resolution and excellent peak symmetry, enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection), which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.
Condition
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN 80% |
| Buffer | none |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Description
| Class of Compounds | Acid, Sugar |
| Analyzing Compounds | Lactic Acid, Taurine, Myo-Inositol |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Myo-Inositol
Taurine
Retention of Taurine on Obelisc N Column
July 3, 2013
Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the Obelisc N HILIC/mixed-mode column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.
| Column | Obelisc N, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Taurine |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsZwitterion
Retention of Taurine on SHARC Column
July 3, 2013
Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the SHARC 1 hydrogen-bonding column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.
| Column | Sharc 1, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/MeOH |
| Buffer | Formic Acid – 0.1%, AmFm – 0.01% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Taurine |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsZwitterion
HPLC Method for Analysis of Taurine on Primesep D Column
December 6, 2007
Taurine (2-aminoethanesulfonic acid) is organic zwitterionic compound. Taurine is a non-essential sulfur-containing amino acid that functions with glycine and gamma-aminobutyric acid as a neurotransmitter. Taurine is incorporated into one of the most abundant bile acids, chenodeoxycholic acid, where it serves to emulsify dietary lipids in the intestine, promoting digestion. It is used in food and pharmaceutical formulations. High polarity and zwitterionic nature of taurine complicates analysis of this compound by reverse-phase chromatography. Two methods for the analysis of taurine are developed on mixed-mode columns. Taurine is retained on Primesep A column by HILIC cation-exchange mechanism and on Primesep D column by HILIC anion-exchange mechanism. Method can be used for fast and effective quantitation of taurine in various products including energy drinks. Method requires ELSD or LC/MS detection due to lack of UV activity for taurine.
Application Column
Primesep D
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Zwitterion
HPLC Separation of Taurine on Primesep A
December 6, 2007
Taurine (2-aminoethanesulfonic acid) is organic zwitterionic compound. Taurine is a non-essential sulfur-containing amino acid that functions with glycine and gamma-aminobutyric acid as a neurotransmitter. Taurine is incorporated into one of the most abundant bile acids, chenodeoxycholic acid, where it serves to emulsify dietary lipids in the intestine, promoting digestion. It is used in food and pharmaceutical formulations. High polarity and zwitterionic nature of taurine complicates analysis of this compound by reverse-phase chromatography. Two methods for the analysis of taurine are developed on mixed-mode columns. Taurine is retained on Primesep A column by HILIC cation-exchange mechanism and on Primesep D column by HILIC anion-exchange mechanism. Method can be used for fast and effective quantitation of taurine in various products including energy drinks. Method requires ELSD or LC/MS detection due to lack of UV activity for taurine.
Application Column
Primesep A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Zwitterion
