| CAS Number | 87-89-8 |
|---|---|
| Molecular Formula | C6H12O6 |
| Molecular Weight | 180.16 |
| InChI Key | CDAISMWEOUEBRE-UHFFFAOYSA-N |
| LogP | -3.7 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Myo-Inositol
February 5, 2026
If you are looking for optimized HPLC method to analyze Myo-Inositol check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Determination of Myo-Inositol on Primesep S2 Column
May 7, 2025
Separation type: Liquid Chromatography HILIC
HPLC Method for Analysis Myo-Inositol on Primesep S2 Column by SIELC Technologies
Myo-Inositol
Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.
The myo-inositol can be effectively retained using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN), without the need for a buffer.
This method offers high resolution and excellent peak symmetry, enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection), which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.
Condition
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN 75% |
| Buffer | none |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Description
| Class of Compounds | Sugar |
| Analyzing Compounds | Myo-Inositol |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Simultaneous Determination of Lactic Acid, Taurine, and Myo-Inositol on Primesep S2 Column
May 6, 2025
Separation type: Liquid Chromatography HILIC
HPLC Method for Analysis Lactic Acid, Taurine, Myo-Inositol on Primesep S2 Column by SIELC Technologies
Lactic Acid
Commonly used in food preservation, cosmetics, pharmaceuticals, and as a biomarker in physiological studies (e.g., exercise and metabolism).
Taurine
Widely present in energy drinks and supplements; important in cardiovascular function, development of the nervous system, and cellular protection.
Myo-Inositol
Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.
The mixture of lactic acid, taurine, and myo-inositol can be effectively retained and separated using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN), without the need for a buffer.
The mixture consists of lactic acid, an organic α-hydroxy acid; taurine, a sulfonic acid amino acid derivative; and myo-inositol, a polyol (sugar alcohol), representing diverse chemical classes with varying polarities and functional groups.
This method offers high resolution and excellent peak symmetry, enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection), which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.
Condition
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN 80% |
| Buffer | none |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Description
| Class of Compounds | Acid, Sugar |
| Analyzing Compounds | Lactic Acid, Taurine, Myo-Inositol |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Myo-Inositol
Taurine
