| CAS Number | 87-89-8 |
|---|---|
| Molecular Formula | C6H12O6 |
| Molecular Weight | 180.16 |
| InChI Key | CDAISMWEOUEBRE-UHFFFAOYSA-N |
| LogP | -3.7 |
| Synonyms |
|
Applications:
HPLC Method for Determination of Myo-Inositol on Primesep S2 Column
May 7, 2025
Separation type: Liquid Chromatography HILIC
HPLC Method for Analysis Myo-Inositol on Primesep S2 Column by SIELC Technologies
Myo-Inositol
Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.
The myo-inositol can be effectively retained using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN), without the need for a buffer.
This method offers high resolution and excellent peak symmetry, enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection), which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.
Condition
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN 75% |
| Buffer | none |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Description
| Class of Compounds | Sugar |
| Analyzing Compounds | Myo-Inositol |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Simultaneous Determination of Lactic Acid, Taurine, and Myo-Inositol on Primesep S2 Column
May 6, 2025
Separation type: Liquid Chromatography HILIC
HPLC Method for Analysis Lactic Acid, Taurine, Myo-Inositol on Primesep S2 Column by SIELC Technologies
Lactic Acid
Commonly used in food preservation, cosmetics, pharmaceuticals, and as a biomarker in physiological studies (e.g., exercise and metabolism).
Taurine
Widely present in energy drinks and supplements; important in cardiovascular function, development of the nervous system, and cellular protection.
Myo-Inositol
Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.
The mixture of lactic acid, taurine, and myo-inositol can be effectively retained and separated using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN), without the need for a buffer.
The mixture consists of lactic acid, an organic α-hydroxy acid; taurine, a sulfonic acid amino acid derivative; and myo-inositol, a polyol (sugar alcohol), representing diverse chemical classes with varying polarities and functional groups.
This method offers high resolution and excellent peak symmetry, enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection), which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.
Condition
| Column | Primesep S2, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN 80% |
| Buffer | none |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Description
| Class of Compounds | Acid, Sugar |
| Analyzing Compounds | Lactic Acid, Taurine, Myo-Inositol |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Myo-Inositol
Taurine
