HPLC Method for Determination of Myo-Inositol on Primesep S2 Column

Separation type: Liquid Chromatography HILIC

HPLC Method for Analysis Myo-Inositol on Primesep S2 Column by SIELC Technologies

HPLC Method for Analyzing Myo-Inositol on Primesep S2 Column by SIELC Technologies

Myo-Inositol

Used in the treatment of polycystic ovary syndrome (PCOS), neurological conditions, and as a dietary supplement for metabolic and reproductive health.

The myo-inositol can be effectively retained using a mixed-mode Primesep S2 column with a mobile phase consisting of 80% acetonitrile (MeCN), without the need for a buffer.
This method offers high resolution and excellent peak symmetry, enabling accurate quantification of all three analytes. Detection is performed using ELSD (Evaporative Light Scattering Detection), which provides reliable sensitivity for non-UV-active or weakly absorbing compounds.

Condition

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN 75%
Buffernone
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50°C,
 with a gas flow rate of 1.6 Standard Liters per Minute (SLM)

Description

Class of CompoundsSugar
Analyzing CompoundsMyo-Inositol

Application Column

Primesep S2

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Myo-Inositol
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.