Zwitterion

Synonyms

    Applications:

    Retention of Taurine on Obelisc N Column

    2013-07-03

    Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the Obelisc N HILIC/mixed-mode column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.

    Condition

    Column Obelisc N, 4.6×50 mm, 5 µm, 100A
    Mobile Phase MeCN
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Taurine

     

    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Obelisc N

    SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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    Retention of Taurine on SHARC Column

    2013-07-03

    Taurine, or 2-aminoethanesulfonic acid, is a very polar zwitter-ionic compound. Taurine is used as an additive for various nutrition composition. The polar and zwitter-ionic nature of taurine prevent it from analysis by RP chromatography, in addition to that, taurine is not UV active and cannot be monitored by UV. The analytical method for analysis of taurine was developed on the SHARC 1 hydrogen-bonding column. This method with some modifications can be used for the analysis of taurine in complex mixtures with a ELSD or a LC/MS detector.

    Condition

    Column Sharc 1, 4.6×50 mm, 5 µm, 100A
    Mobile Phase MeCN/MeOH
    Buffer Formic Acid – 0.1%, AmFm – 0.01%
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Amino sulfonic acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Taurine

     

    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    SHARC 1

    The SHARC™ family of innovative columns are are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding is an interaction (attraction) of bound hydrogen atom to the molecules with electronegative atoms such as oxygen, nitrogen, and fluorine.

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    HPLC Separation of Glutamic Acid and GABA

    2011-07-08

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    Glutamic acid and GABA are neurotransmitters. Glutamic acid and GABA are non-essential amino acids. They are hydrophilic and zwitter-ionic in nature . At lower pH, carboxylic acid groups of amino acids are not ionized, making them more hydrophobic and basic. Underivatized glutamic acid and GABA were retained and separated on a Primesep 100 column using ACN/water/TFA mobile phase. Amino acids can be monitored by low UV or ELSD/CAD. Retention is provided by reversed-phase and cation-exchange mechanism. Method can be used for analysis of underivatized amino acids in various matrices including supplements, vitamin and other complex mixtures. various mobile phase can be used with corresponding detection techniques.

    Condition

    Column Primesep 100, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 10/90%
    Buffer TFA – 0.1%
    Flow Rate 1.0 ml/min
    Detection UV, 215 nm, ELSD

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Glutamic acid, GABA

     

    Application Analytes:
    Glutamic Acid
    Zwitterion
    gamma-Aminobutyric Acid (GABA)

    Application Detection:
    UV Detection
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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    HPLC Separation of Morpholino Sulfates

    2010-11-21


    MOPS and MES are buffers used in biology and biochemistry. They consist of a morpholine ring and propanesulfonic acid. Both molecules are zwitter-ionic and very polar. No retention can be achieved on reversed-phase columns. MOPS and MES are analyzed in HILIC mode on a Primesep N HPLC column. Other biological buffers can be analyzed with this general method. Biological buffers are not UV active and can be monitored by ELSD, CAD or LC/MS.

    Application Analytes:
    2-Morpholinoethanesulfonic Acid (MES)
    4-Morpholinepropanesulfonic Acid (MOPS)
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep N

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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    HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds

    2010-04-10


    Primesep mixed-mode columns are designed to separate basic, acidic, neutral and zwitter-ionic compounds. Elution of these compounds is facilitated by acetonitrile and ions in the mobile phase. In all cases the retention time on mixed-mode columns is significantly longer than on traditional reverse-phase columns. Presence of cation-exchange mechanism in Primesep 100 column allows to achieve better retention and peak shape for analytes like phenylalanine and benzylamine. Various buffers can be used along with different detection techniques. This HPLC method can be adopted as a universal approach for analysis of basic, acidic and zwitter-ionic compounds in complex mixtures and formulations. Compounds can be monitored by UV, ELSD, CAD and LC/MS.

    Application Analytes:
    Benzoic Acid
    Benzylamine
    Phenylalanine
    Zwitterion

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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    Comparison of Obelisc N, SeQuant?® HILIC, and Bare Silica

    2010-03-03


    Obelisc N mixed-mode columns provide different selectivity than other HILIC columns — presence of ion-exchange mechanism contributes to a different selectivity. Depending on the pH of the mobile phase, ion-exchange mechanism can be enhanced or suppressed. Two amino acids (glycine and glycylglycine), two basic compounds (triethanolamine and TRIS) and two zwitter-ionic compounds (MOPS and MES) are separated by combination of HILIC and ion-exchange mechanisms. Compounds elution can be monitored by Evaporative Light-Scattering Detector (ELSD), Corona (CAD), LC/MS or other detection techniques.

    Application Analytes:
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Obelisc N

    SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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    HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N

    2010-03-03


    GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.

    Condition

    Column Obelisc N,  4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Acid
    Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)
    Application Analytes:
    Zwitterion
    alpha-Aminobutyric Acid
    beta-Aminobutyric Acid
    gamma-Aminobutyric Acid (GABA)

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Obelisc N

    SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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    Effect of Buffer on HPLC Separation of Buffers

    2008-08-22

    HEPES, CAPS, MES and MOPS are zwitterionic organic chemical buffering agents. These are very polar compounds which are not retained by traditional reverse phase chromatography. These compounds are zwitterions in nature and can be separated by mixed-mode hydrophilic interaction chromatography on Obelisc N column. Retention is achieved by combination of HILIC and ion-exchange mechanisms. These buffering agents do not have UV active groups, but can be analyzed with ESLD, LC/MS, and CAD detection.

    Application Analytes:
    2-Morpholinoethanesulfonic Acid (MES)
    4-Morpholinepropanesulfonic Acid (MOPS)
    CAPS (3-(Cyclohexylamino)propanesulfonic acid)
    HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Obelisc N

    SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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    HPLC Separation of Taurine

    2007-12-06

    Taurine (2-aminoethanesulfonic acid) is organic zwitterionic compound. Taurine is a non-essential sulfur-containing amino acid that functions with glycine and gamma-aminobutyric acid as a neurotransmitter. Taurine is incorporated into one of the most abundant bile acids, chenodeoxycholic acid, where it serves to emulsify dietary lipids in the intestine, promoting digestion. It is used in food and pharmaceutical formulations. High polarity and zwitterionic nature of taurine complicates analysis of this compound by reverse-phase chromatography. Two methods for the analysis of taurine are developed on mixed-mode columns. Taurine is retained on Primesep A column by HILIC cation-exchange mechanism and on Primesep D column by HILIC anion-exchange mechanism. Method can be used for fast and effective quantitation of taurine in various products including energy drinks. Method requires ELSD or LC/MS detection due to lack of UV activity for taurine.

    Application Analytes:
    Taurine
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep D

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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    Retention Characteristics of Betaine in Mixed-Mode HPLC

    2005-05-05

    HPLC Application: Retention Characteristics of Betaine in Mixed-Mode HPLC

    The retention of the zwitterion betaine on Primesep 200 demonstrates the tunability of Primesep columns. Retention of betaine is altered by acid type, amount of acid in the mobile phase, and the amount of organic solvent. Typical reversed-phase columns do not show this tunability with simple mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

    Application Analytes:
    Betaine
    Quaternary Amines
    Zwitterion

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Primesep 200

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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    Primesep C

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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    TFA Concentration Effect on Retention of Tricine

    2004-04-05

    Primesep A retains the hydrophilic zwitterion tricine by a combination of polar interactions and ion exchange. Tricine is not retained by traditional reversed-phase chromatography. The retention on Primesep A is adjustable by changing the concentration of TFA in the mobile phase. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

    Application Analytes:
    Tricine
    Zwitterion

    Application Detection:
    ELSD/MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.