HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N

GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.


Column Obelisc N,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Acid
Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)

Application Analytes:
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)