CAS Number | 107-43-7 |
---|---|
Molecular Formula | C5H11NO2 |
Molecular Weight | 117.148 |
InChI Key | KWIUHFFTVRNATP-UHFFFAOYSA-N |
LogP | -2.72 |
Synonyms |
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In this HPLC method, betaine and carboxymethylcysteine are separated by HILIC mixed-mode mechanism. Betaine and carboxymethylcysteine are very hydrophilic compounds which are not retained on reverse phase columns. Compounds are separated by combination of HILIC and ion-exchange mechanisms. In HIILIC mode, Obelisc N columns allow operating at lower organic concentration for improved solubility of analytes. Method can be used for quantitative or qualitative analysis of betaine and carboxymethylcysteine using ESLD or LC/MS detection. Betaine in chemistry is any neutral chemical compound with a positively charged cationic functional group such as ammonium ion or phosphonium ion (generally: onium ions) which bears no hydrogen atom and with a negatively charged functional group such as a carboxylate group which may not be adjacent to the cationic site. Historically the term was reserved for tetramethylglycine only.
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsThe retention of the zwitterion betaine on Primesep 200 demonstrates the tunability of Primesep columns. Retention of betaine is altered by acid type, amount of acid in the mobile phase, and the amount of organic solvent. Typical reversed-phase columns do not show this tunability with simple mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsThe Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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