Tricine

CAS Number	5704-04-1
Molecular Formula	C6H13NO5
Molecular Weight	179.172
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
LogP	-1.67
Synonyms	Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- N-[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine 5704-04-1 EINECS 227-193-6 NSC 369995 UNII-W12LH4V8V3 N-(tri(hydroxymethyl)methyl)glycine N-tris(hydroxymethyl)methylglycine Tricine

Applications:

TFA Concentration Effect on Retention of Tricine

2004-04-05

View Application

Primesep A retains the hydrophilic zwitterion tricine by a combination of polar interactions and ion exchange. Tricine is not retained by traditional reversed-phase chromatography. The retention on Primesep A is adjustable by changing the concentration of TFA in the mobile phase. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

Application Column

Primesep A

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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Tricine
Zwitterion

ELSD Detection

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.