HPLC Determination of Dicyandiamide on Sharc 1 Column

HPLC Method for Dicyandiamide on SHARC 1 by SIELC Technologies

HPLC Determenation of Dicyandiamide on Sharc 1 Column_1200

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dicyandiamide 

Dicyandiamide, also known as cyanoguanidine, has a C₂H₄N₄ chemical formula. It is a white crystalline powder that has a variety of uses from agricultural to mechanical. Though, typically it is found in fertilizers as a nitrification inhibitor. It slows down nitrogen release and and reduces losses from soil.

Dicyandiamide can be retained and analyzed using the SHARC 1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using CAD.

Condition
Column SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 97.8%
Buffer AmFm 0.2%,  Formic Acid – 2%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description
Class of Compounds Amide, Hydrophilic, Ionizable
Analyzing Compounds Dicyandiamide

Application Column

SHARC 1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Dicyandiamide
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.