HPLC Determination of Allantoin on SHARC 1 Column

HPLC.cloud View on hplc.cloud Allantoin is a 5-ureidohydantoin or glyoxyldiureide with the chemical formula C4H6N4O3 and is found in plants like chamomile, wheat sprouts, tobacco seed, sugar beet and comfrey.  Allantoin can be retained using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism with isocratic method by using a mobile phase of acetonitrile (ACN) and methanol (MeOH).  Allantoin can be monitored by low UV (210 nm), ELSD, CAD  or LC/MS.  


Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH - 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 210 nm


Class of Compounds Hydrophilic, Amide, Amine
Analyzing Compounds Allantoin
Application Analytes: