HPLC Determination of Allantoin on SHARC 1 Column

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Allantoin is a 5-ureidohydantoin or glyoxyldiureide with the chemical formula C4H6N4O3 and is found in plants like chamomile, wheat sprouts, tobacco seed, sugar beet and comfrey.  Allantoin can be retained using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism with isocratic method by using a mobile phase of acetonitrile (ACN) and methanol (MeOH).  Allantoin can be monitored by low UV (210 nm), ELSD, CAD  or LC/MS.



Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH – 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 210 nm



Class of Compounds
Hydrophilic, Amide, Amine
Analyzing Compounds Allantoin


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.