HPLC Method for Imidazolidinyl urea on SHARC 1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Imidazolidinyl urea.
Imidazolidinyl urea is a preservative with the chemical formula C11H16N8O8. used in cosmetics as due to its antimicrobial properties. It is active against a broad spectrum of gram positive and gram negative bacteria, yeast and molds.
Imidazolidinyl urea can be retained in HPLC using a SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The isocratic method uses a mobile phase of acetonitrile (ACN) and water. Imidazolidinyl urea can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
| Column | SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/MeOH – 90/10% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds |
Hydrophilic, Amide, Amine |
| Analyzing Compounds | Imidazolidinyl urea |
Application Column
SHARC 1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Imidazolidinyl ureaApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
