HPLC Determination of Imidazolidinyl urea on SHARC 1 Column

Separation type: Liquid Chromatography HILIC

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High Performance Liquid Chromatography (HPLC) Method of Imidazolidinyl urea.
Imidazolidinyl urea is used in cosmetics as a preservative due to its antimicrobial properties. It is active against a broad spectrum of gram positive and gram negative bacteria, yeast and molds. It can be retained in HPLC using a SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The isocratic method uses a mobile phase of acetonitrile (ACN) and water. Imidazolidinyl urea can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.


Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH - 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 210 nm


Class of Compounds Hydrophilic, Amide, Amine
Analyzing Compounds Imidazolidinyl urea
Application Analytes:
Imidazolidinyl urea