Separation type: Liquid Chromatography HILIC
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High Performance Liquid Chromatography (HPLC) Method of Imidazolidinyl urea.
Imidazolidinyl urea is used in cosmetics as a preservative due to its antimicrobial properties. It is active against a broad spectrum of gram positive and gram negative bacteria, yeast and molds. It can be retained in HPLC using a SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The isocratic method uses a mobile phase of acetonitrile (ACN) and water. Imidazolidinyl urea can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
|Column||Sharc 1, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/MeOH - 90/10%|
|Flow Rate||1.0 ml/min|
|Detection||UV 210 nm|
|Class of Compounds||Hydrophilic, Amide, Amine|
|Analyzing Compounds||Imidazolidinyl urea|