HPLC Determination of Imidazolidinyl urea on SHARC 1 Column

Separation type: Liquid Chromatography HILIC

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High Performance Liquid Chromatography (HPLC) Method of Imidazolidinyl urea.

Imidazolidinyl urea is used in cosmetics as a preservative due to its antimicrobial properties. It is active against a broad spectrum of gram positive and gram negative bacteria, yeast and molds. It can be retained in HPLC using a SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The isocratic method uses a mobile phase of acetonitrile (ACN) and water. Imidazolidinyl urea can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.


Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH – 90/10%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 210 nm



Class of Compounds
Hydrophilic, Amide, Amine
Analyzing Compounds Imidazolidinyl urea


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
Imidazolidinyl urea
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.