Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)
| Column | Sharc 1, 4.6×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/MeOH – 90/10% |
| Buffer | H2SO4 – 0.2 |
| Flow Rate | 3 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Paraquat, Diquat |
Application Column
Application Analytes:
DiquatParaquat
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
