HPLC Separation of Mixture of DMF and DMSO

Dimethylformamide, DMF, is primarily used as an organic solvent and is miscible with water and most other organic liquids. Dimethyl sulfoxide (DMSO) is also a solvent. It can dissolve both polar and nonpolar compounds and is miscible with water and other organic liquids. Both compounds can be retained and separated using anhydrous (water-free) conditions using HPLC by SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase without the need for a buffer. UV detection used at 220nm.


Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 98-85 %, 10 min
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 220 nm



Class of Compounds
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds DMF, DMSO


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
DMF (Dimethylformamide)
DMSO (Dimethyl sulfoxide)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.