HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding


Column Sharc 1, 3.2x50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 100/0 - 30/70%
Buffer Formic Acid - 0.5% AmFm 0.05% 7 min
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Application Analytes:
DOPAC (Dihydroxyphenylacetic Acid)
Homovanillic Acid

Application Detection:
UV Detection