HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding


Column Sharc 1, 3.2×50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH – 100/0 – 30/70%
Buffer Formic Acid – 0.5% AmFm 0.05% 7 min
Flow Rate 1.0 ml/min
Detection UV, 270 nm



Class of Compounds
Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
DOPAC (Dihydroxyphenylacetic Acid)
Homovanillic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.