| Column | Sharc 1, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN/MeOH – 100/0 – 30/70% |
| Buffer | Formic Acid – 0.5% AmFm 0.05% 7 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | DOPAC, Homovanillic Acid, Dopamine |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsApplication Analytes:
DOPAC (Dihydroxyphenylacetic Acid)Dopamine
Homovanillic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
