| CAS Number | 306-08-1 |
|---|---|
| Molecular Formula | C9H10O4 |
| Molecular Weight | 182.176 |
| InChI Key | QRMZSPFSDQBLIX-UHFFFAOYSA-N |
| LogP | 0.33 |
| Synonyms |
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Applications:
Generic Screening Method for Complex Mixtures on Primesep 200
October 15, 2015
| Column | Primesep 200, 4.6*150 mm 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 215 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethylparaben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil
HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column
July 10, 2012
Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.
Application Columns: Primesep 100
Application compounds: Dopac, Homovanillic Acid, Dopamine
Detection technique: UV, LC/MS, ELSD/CAD
| Column | Primesep 100, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | DOPAC, Homovanillic Acid, Dopamine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDopamine
Homovanillic Acid
HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 200 Column
July 10, 2012
Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.
Application Columns: Primesep 100, Primesep 200
Application compounds: Dopac, Homovanillic Acid, Dopamine
Detection technique: UV, LC/MS, ELSD/CAD
| Column | Primesep 200, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | DOPAC, Homovanillic Acid, Dopamine |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Dopamine
Homovanillic Acid
HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding
June 18, 2012
| Column | Sharc 1, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN/MeOH – 100/0 – 30/70% |
| Buffer | Formic Acid – 0.5% AmFm 0.05% 7 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | DOPAC, Homovanillic Acid, Dopamine |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsDopamine
Homovanillic Acid
Separation of Serotonin, Dopamine, and Related Compounds
August 22, 2008
Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).
| Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 4.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Homovanillic acid, Dihydroxyphenyl acetic acid, Serotonin, Dopamine, Epinephrine, Hydroxytryptophan, DOPA |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsDOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin
HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids
November 21, 2006
| Column | Newcrom B , 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TFA, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Acid, Monocarboxylic acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Vanilmandelic, Hydroxyindoleacetic, Homovanillic Acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHomovanillic Acid
Vanilmandelic Acid
