HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column

  Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.   Application Columns: Primesep 100 Application compounds: Dopac, Homovanillic Acid, Dopamine Detection technique: UV, LC/MS, ELSD/CAD


Column Primesep 100, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
Application Analytes:
DOPAC (Dihydroxyphenylacetic Acid)
Homovanillic Acid