HPLC Method for Analysis of Clotrimazole, Benzyl alcohol, Ethylparaben on Primesep 200 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Clotrimazole, Benzyl alcohol, Ethylparaben

Clotrimazole is an antifungal medication widely used in the treatment of fungal infections.

Description:

• Classification: Clotrimazole belongs to the class of medications called imidazole antifungals.

Uses:

• Topical: It’s commonly used to treat skin infections such as athlete’s foot, jock itch, ringworm, and other fungal skin infections (candidiasis).
• Vaginal: As a vaginal cream or tablet, it’s used to treat vaginal yeast infections.
• Otic (Ear): Clotrimazole solution can be used to treat fungal ear infections.

Mechanism of Action:

• Clotrimazole works by inhibiting the synthesis of ergosterol, a vital component of fungal cell membranes. When ergosterol synthesis is inhibited, it leads to structural and functional impairment of the fungal cell membrane, thereby killing the fungus or inhibiting its growth.

Clotrimazole belongs to the class of chemical compounds known as imidazoles. Specifically, it’s an antifungal compound used to treat fungal infections. Imidazoles work by inhibiting the enzyme lanosterol 14α-demethylase, which is necessary for the synthesis of ergosterol, an essential component of fungal cell membranes. When ergosterol synthesis is disrupted, it leads to alterations in the cell membrane’s permeability and, eventually, fungal cell death.

Other antifungal agents in the imidazole class include miconazole, econazole, and ketoconazole, among others. These are commonly used in various formulations to treat a range of fungal infections, including athlete’s foot, ringworm, and yeast infections.

Clotrimazole can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.

HPLC Method for Ethylparaben, Methylparaben sodium, Propylparaben sodium, Methylparaben, Parabens, Propylparaben on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylparaben, Methylparaben sodium, Propylparaben sodium, Methylparaben, Parabens, Propylparaben.

Methylparaben, also known as Methyl 4-hydroxybenzoate, is a paraben with the chemical formula C₈H₈O₃. It is used as a preservative in food, cosmetics, and pharmaceuticals as it is said to have antimicrobial and antifungal properties. It is considered safe for use in low concentrations, but it may cause irritation or contact dermatitis in rare cases and for those who are allergic.

Ethylparaben is an ethyl ester of p-hydroxybenzoic acid with the chemical formula C₉H₁₀O₃. It is used as a preservative in food, cosmetics, and pharmaceuticals due to it’s antimicrobial and antifungal properties. There are ongoing debates in regards to it’s safety as it relates to human consumption,

Propylparaben is a n-propyl ester with the chemical formula C₁₀H₁₂O₃. It is used as a preservative in food, cosmetics, and personal care products due to it’s antimicrobial properties. Studies show that it absorbs through the skin and remains in the body, leading to the European Union banning it. California had banned it for use in food, but not in other uses.

You can find detailed UV spectra of Methylparaben and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Ethylparaben and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Propylparaben and information about its various lambda maxima by visiting the following link.

Ethylparaben, Methylparaben sodium, Propylparaben sodium, Methylparaben, Parabens, Propylparaben can be retained and analyzed using the Newcrom AH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Polar amino acids are very often used as components of vitamin and supplement composition. Analysis of such complex composition is a challenging task. In this application, 5 amino acids (asparagine, glutamic acid, proline and arginine) and two preservatives (methyl paraben and propyl paraben) are separated on a Primesep 100 reversed-phase cation-exchange column with LC/MS compatible mobile phase. Method does not require ion-pairing reagent in the mobile phase. Compounds are monitored by ELSD and UV. Method is validated for quantitation of underivatized amino acids in complex mixtures. The method is simple and robust and can be used for analysis of various vitamin formulations.