Lysine

Lysine

CAS Number56-87-1
Molecular FormulaC6H14N2O2
Molecular Weight146.190
InChI KeyKDXKERNSBIXSRK-YFKPBYRVSA-N
LogP-3.05
Synonyms
  • Lysine
  • L-Lysine
  • 56-87-1
  • 4-04-00-02717
  • L-Lysine
  • (+)-S-Lysine
  • (S)-2,6-Diaminohexanoic acid
  • (S)-Lysine
  • (S)-α,ε-Diaminocaproic acid
  • 2,6-Diaminohexanoic acid
  • Aminutrin
  • Aminutrin, 6-amino-
  • Hexanoic acid, 2,6-diamino-, (S)-
  • h-Lys-oh
  • L-(+)-LYSINE
  • L-2,6-Diaminocaproic acid
  • L-lisina
  • L-Lysin
  • L-Norleucine, 6-amino-
  • Lysine acid
  • LYSINE, L-
  • Malandil
  • α-Lysine
  • BRN 1722531
  • 2,6-Diaminohexanoic acid, (S)-
  • EINECS 200-294-2
  • Lysinum
  • UNII-K3Z4F929H6
  • (2S)-2,6-diaminohexanoic acid
  • (S)-2,6-Diaminohexanoate
  • (S)-2,6-diamino-Hexanoate
  • (S)-2,6-diamino-Hexanoic acid
  • (S)-a,e-Diaminocaproate
  • (S)-a,e-Diaminocaproic acid
  • (S)-alpha,epsilon-diaminocaproic acid
  • 2,6-Diaminohexanoate
  • 6-Amino-Aminutrin
  • 6-Amino-L-Norleucine
  • K
  • L-2,6-Diainohexanoate
  • L-2,6-Diainohexanoic acid
  • L-2,6-Diaminocaproate
  • L-Lys
  • Lys
  • a-Lysine
  • alpha-Lysine
  • 1150316-18-9
  • 280114-50-3
  • 48050-57-3
  • 6899-06-5

Applications:

HPLC Method for Separation of  Lysine, Dilysine and Trilysine on BIST B+ Column

March 27, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Lysine, Dilysine, Trilysine on BIST B+ by SIELC Technologies

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Lysine, Dilysine, Trilysine on on BIST B+ by SIELC Technologies

Lysine, dilysine, and trilysine refer to compounds related to the amino acid lysine.

1. Lysine (L-Lysine):

  • Type: Lysine is an essential amino acid.
  • Structure: It has an α-amino group (which is in the protonated –NH3+ form under biological conditions), an α-carboxylic acid group (which is in the deprotonated –COO− form under physiological conditions), and a side chain with a functional group that is a basic, charged (at physiological pH) amino group.
  • Role in the Body: As an amino acid, lysine is a building block for proteins. It’s crucial for health, but the body can’t produce it, so it must come from the diet.
  • Uses: Lysine has been used in supplements to improve athletic performance, support immune health, and more. It’s also integral in the production of carnitine, a nutrient responsible for converting fatty acids into energy and helping to lower cholesterol.

2. Dilysine:

  • Type: This refers to a molecule composed of two lysine units.
  • Structure: The two lysine amino acids can be joined via a peptide bond between the carboxyl group of one lysine molecule and the amino group of another, forming a dipeptide. The resulting structure still features the basic side chains of the lysine residues.
  • Potential Roles and Uses: While not commonly discussed outside of scientific research, such compounds could play roles in biological signaling or act as building blocks for more complex molecules. They may also be used in certain types of biochemical research or have potential therapeutic properties.

3. Trilysine:

  • Type: This compound comprises three lysine molecules.
  • Structure: Similar to dilysine, but with three lysine units connected by peptide bonds, resulting in a tripeptide. The basic side chains characteristic of lysine would still be present, and the molecule would carry multiple positive charges under physiological conditions due to these groups.
  • Potential Roles and Uses: Trilysine is even less common in general discussion and is primarily a subject of scientific study. It could play a role in biochemical research or be explored for various potential applications, perhaps related to its charge and interaction with other molecules.

For all these compounds, especially dilysine and trilysine, their relevance is often in biochemical research. Scientists may study such peptides to understand cellular mechanisms, explore physiological processes, or develop new materials or medications. The specific properties of these compounds, such as their charge and their ability to participate in various biochemical interactions, can make them valuable for certain applications.

Lysine, dilysine, and trilysine can be retained, separated and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) or Methanol (MeOH), and a sulfuric acid as a buffer. This analysis method can be detected using UV at 205 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Lysine, Dilysine, Trilysine

Condition

ColumnBIST B+, 4.6 x 50 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN or MeOH
Buffer H2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 205 nm

Description

Class of CompoundsAmino acid
Analyzing CompoundsLysine, Dilysine, Trilysine

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Dilysine
Lysine
Trilysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Glucose and Lysine on Primesep S Column

January 25, 2023

HPLC Method for Separation of Glucose and Lysine on Primesep S by SIELC Technologies

 

HPLC Method for Separation of Glucose and Lysine on Primesep S  by SIELC Technologies
HPLC Method for Separation of Glucose and Lysine on Primesep S Column by SIELC Technologies

Lysine is an essential amino acid typically found in meat, and also cereal grains. It can be manufactured on a large scale by fermenting glucose, one of the most abundant sugars in the world. These two biological compounds can be retained, separated, and analyzed on a normal-phase Primesep S column with a simple isocratic mobile phase consisting of Acetonitrile (MeCN), water (H2O), and Sulfuric acid (H2SO4). This analysis method can be detected via Refraction Index (RI) Detection. 

High Performance Liquid Chromatography (HPLC) Method for Separation of Glucose and Lysine

Condition

ColumnPrimesep S , 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 75/25%
BufferH2SO4 – 0.5%
Flow Rate1.0 ml/min
DetectionRefraction Index
Peak Retention Time4.81 min

Description

Class of CompoundsDrug
Analyzing CompoundsGlucose, Lysine

Application Column

Primesep S2

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Glucose
Lysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Arginine, Lysine and Histidine Amino Acids on BIST B+

November 30, 2022

HPLC Method for Analysis of Arginine, Lysine and Histidine Amino Acids on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Arginine, Lysine and Histidine Amino Acids on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Arginine, Lysine and Histidine Amino Acids

Basic amino acids, like Arginine, Lysine, and Histidine, can be difficult to separate and retain in typical reverse-phase chromatography. Arginine is a naturally-occurring, essential amino acid typically found in meat, poultry, and fish. Lysine is another essential amino acid typically found in meat, and also cereal grains. A third essential amino acid, Histidine, is also typtically found in meat, poultry, and also cereal grains (like rice, wheat, and rye). Using SIELC’s newly introduced BIST™ method, these three essential amino acids, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged amino acid analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Arginine, Lysine, and Histidine can be retained and UV detected at 210 nm.


Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Peak Retention Time6.2, 8.0, 9.9 min

Description

Class of CompoundsAmino acid
Analyzing CompoundsArginine, Lysine, Histidine

Application Column

BIST B

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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BIST B+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Arginine
Histidine
Lysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Nine Essential Amino acids and Arginine on Newcrom AH Column

September 25, 2020


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Essential amino acids cannot be made by the body. As a result, they must come from food.
The 9 essential amino acids are: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 5%
Buffer Gradient Formic Acid – 3-9%, 20 min
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), Lysine (Lys/K)
, L- Tryptophan (Trp/W), L-Arginine (Arg/R)

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Arginine
Histidine
Isoleucine
L-Threonine
Leucine
Lysine
Methionine
Phenylalanine
Tryptophan
Valine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Lysine on Primesep 200 column in MS-compatible conditions

September 16, 2019


Lysine is an essential amino acid used in the synthesis of proteins. In biological conditions, it is a basic, charged molecule. Lysine can be retained on Primesep 200 mixed-mode column which has embedded weak acidic ion-pairing groups for retention of basic analytes in HPLC. The mobile phase consisting of acetonitrile (ACN) and water with ammonium formate (AmFm) makes this method analysis MS-compatible.

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFM pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona), MS- compatible phase

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Amino Acid
Analyzing Compounds Lysine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
D-Lysine
Lysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of D-Glucose and L-Lysine on Primesep S Column

February 7, 2019

 

Condition

Column Primesep S, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 85-60%, 10 min 2 min hold
Buffer Gradient AmFm pH 3.0, 10-80 mM, 10 min 2 min hold
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description

Class of Compounds
Sugar, Amino Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds D-Glucose, L-Lysine

 

Application Column

Primesep S

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Glucose
Lysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of L-Lysine and L-Arginine

February 28, 2018

l-Lysine , L-Arginine Chr_1106

L-Lysine is an essential amino acid required for growth and tissue repair, it can also help with anxiety. The amino acid is most often found in red meats,beans, fish, and dairy products. L-Arginine is an essential amino acid that is used to treat congestive heart failure, combat fatigue and circulatory diseases. It is also used to stimulate the immune system. Obelisc R is a reverse-phase column. It contains embedded ionic groups and can retain L-Lysine and L-Arginine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer Na2HPO4 pH 6.0 – 25 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, L-Lysine

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Arginine
D-Lysine
Lysine
N-Nitro-L-arginine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Lysine and Arginine from Other Amino Acids

July 10, 2012

 

Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge.

Application Columns: Primesep C
Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 15%
Buffer AmAc pH 5.0- 15 mM
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Small Lysine-based Peptide Oligomers on BIST B+ Column

July 8, 2011

 

HPLC Method for Analysis of Polylysine on BIST™ B+ Column

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 215 nm.

Condition

Column BIST B+, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV 215 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Lysine-based Peptide Oligomers

 

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Lysine
Polylysine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column

July 8, 2011

Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis.

Condition

Column Primesep 200, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Na2HPO4
Flow Rate 1.0 ml/min
Detection UV 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Valine, Leucyne, Isoleycine, Tyrosine, Phenylalanine, Histidine, Lysine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Histidine
Isoleucine
Leucine
Lysine
Phenylalanine
Tyrosine
Valine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of Active Drug and Amino Acids in a Formulation

October 14, 2010


Polar amino acids are very often used as components of vitamin and supplement composition. Analysis of such complex composition is a challenging task. In this application, 5 amino acids (asparagine, glutamic acid, proline and arginine) and two preservatives (methyl paraben and propyl paraben) are separated on a Primesep 100 reversed-phase cation-exchange column with LC/MS compatible mobile phase. Method does not require ion-pairing reagent in the mobile phase. Compounds are monitored by ELSD and UV. Method is validated for quantitation of underivatized amino acids in complex mixtures. The method is simple and robust and can be used for analysis of various vitamin formulations.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD 50C, UV 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Glutamic acid, Aspargine,  Proline, Lysine, Arginine, Methyl paraben, Propyl paraben

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Arginine
Asparagine
Ethylparaben
Glutamic Acid
Lysine
Methylparaben
Proline

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Determination of Lysine in Ibuprofen Lysine Composition

July 16, 2009

Ibuprofen lysine is ibuprofen based medication which is uses lysine as counter-ion of the ibuprofen. The use of lysine makes the drug more soluble in water. Lysine is very hydrophilic compound with low UV activity, while ibuprofen is hydrophobic compound with good UV activity. These properties are making straight analysis of Ibuprofen Lysine (ibuprofen lysinate) a challenging task. Ion-pairing reagent required for retention of lysine in reversed-phase chromatography. Both compounds can be quantitated in one run without use of IP reagent. Because of the huge difference in UV activity of ibuprofen and lysine, a separate method for quantitation of lysine in ibuprofen lysinate might be required. Separation involving switching valve and guard column allows to trap ibuprofen on the guard and wash it away during analysis of lysine. This HPLC method allows to quantitate lysine separately from ibuprofen. Detailed set up of switching valve/guard system is described in our newsletter. Method can be adapted to quantitation of single compound in complex mixtures.

Application Analytes:
Ibuprofen
Lysine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Amino Acids Analysis in Acid Gradient Condition

September 18, 2006

11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

Condition

Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer TFA , gradient  0.05-0.3 % , 25 min
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Aspartic acid, Glutamic acid, Alanine, Valine, Methionine, Isoleucine, Cysteine, Phenylalanine, Histidine, Lysine, Arginine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.