N-Nitro-L-arginine structural formula

CAS Number2149-70-4
Molecular FormulaC6H13N5O4
Molecular Weight219.202
  • N-Nitro-L-arginine
  • N5-(N-Nitrocarbamimidoyl)-L-ornithine
  • L-Ornithine, N5-[imino(nitroamino)methyl]-
  • 2149-70-4
  • L-NNA
  • L-Ornithine, N5-[imino(nitroamino)methyl]-
  • (+)-NG-Nitroarginine
  • L-Arginine, NG-nitro-
  • L-Arginine, ?-nitro-
  • L-NG-Nitroarginine
  • NG-Nitroarginine
  • NG-Nitro-L-arginine
  • Nitroarginine
  • Nitro-L-arginine
  • NSC 53662
  • N?-Nitro-L-arginin
  • N?-nitro-L-arginina
  • N?-Nitro-L-arginine
  • Ornithine, N5-(nitroamidino)-, L-
  • ?-Nitroarginine
  • ?-Nitro-L-arginine
  • Arginine, omega-nitro-
  • Arginine, omega-nitro-, L-
  • Arginine, N(G)-nitro-
  • EINECS 218-418-9
  • N(G)-Methyl-L-arginine
  • omega-Nitroarginine
  • N(G)-Nitroarginine
  • N(G)-Nitro-L-arginine
  • Norvaline, 5-(2-nitroguanidino)-, (+)-
  • Ornithine, N(5)-(nitroamidino)-, L-
  • N(sup G)-Nitro-L-arginine
  • UNII-7O8V7H6P2J
  • 126265-23-4
  • 13855-78-2
  • 38733-00-5


HPLC Method for Analysis of L-Lysine and L-Arginine

February 28, 2018

l-Lysine , L-Arginine Chr_1106

L-Lysine is an essential amino acid required for growth and tissue repair, it can also help with anxiety. The amino acid is most often found in red meats,beans, fish, and dairy products. L-Arginine is an essential amino acid that is used to treat congestive heart failure, combat fatigue and circulatory diseases. It is also used to stimulate the immune system. Obelisc R is a reverse-phase column. It contains embedded ionic groups and can retain L-Lysine and L-Arginine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.


Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer Na2HPO4 pH 6.0 – 25 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm



Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, L-Lysine

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of N-Nitro-L-arginine on Newcrom R1 HPLC column

February 16, 2018
Separation of N-Nitro-L-arginine on Newcrom C18 HPLC column

N-Nitro-L-arginine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.