HPLC Method for Analysis of L-Lysine and L-Arginine

l-Lysine , L-Arginine Chr_1106

L-Lysine is an essential amino acid required for growth and tissue repair, it can also help with anxiety. The amino acid is most often found in red meats,beans, fish, and dairy products. L-Arginine is an essential amino acid that is used to treat congestive heart failure, combat fatigue and circulatory diseases. It is also used to stimulate the immune system. Obelisc R is a reverse-phase column. It contains embedded ionic groups and can retain L-Lysine and L-Arginine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.


Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer Na2HPO4 pH 6.0 – 25 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm



Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, L-Lysine

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.