HPLC Method for Polylysine on BIST B+ by SIELC Technologies

Polylysine is a group of lysine homopolymers with the chemical formula (C₆H₁₂N₂O)_n. Due to its’ high positive charge density, it is often used in drug delivery for complexes with negatively charged macromolecules. They have been especially useful in delivery of DNA and proteins.

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, Polylysine can be retained and UV detected at 205 nm.

Description

HPLC Method for Polylysine on BIST B+ by SIELC Technologies

Polylysine is a group of lysine homopolymers with the chemical formula (C₆H₁₂N₂O)_n. Due to its’ high positive charge density, it is often used in drug delivery for complexes with negatively charged macromolecules. They have been especially useful in delivery of DNA and proteins.

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, Polylysine can be retained and UV detected at 205 nm.

Description

HPLC Method for Analysis of Narrow Fractions of Polylysine on BIST B+ by SIELC Technologies.

Polylysine is a group of lysine homopolymers with the chemical formula (C₆H₁₂N₂O)_n. Due to its’ high positive charge density, it is often used in drug delivery for complexes with negatively charged macromolecules. They have been especially useful in delivery of DNA and proteins.

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as Polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, Polylysine can be retained and UV detected at 210 nm.

Description

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 215 nm.

Polylysine is a small natural homo-polymer made out of amino acid lysine that is produced by bacterial fermentation. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polylysine was successfully analyzed on a Primesep AP column in HILIC/cation-exclusion mode. Cation-exclusion mode on a Primesep AP column allows to obtain sharper peak. Presence of the basic groups on the surface of silica eliminated silanol interactions between basic sites of polylysine and acidic sites of Primesep AP column.