HPLC Determination of Lysine on Primesep 200 column in MS-compatible conditions


Lysine is an essential amino acid used in the synthesis of proteins. In biological conditions, it is a basic, charged molecule. Lysine can be retained on Primesep 200 mixed-mode column which has embedded weak acidic ion-pairing groups for retention of basic analytes in HPLC. The mobile phase consisting of acetonitrile (ACN) and water with ammonium formate (AmFm) makes this method analysis MS-compatible.

Condition
Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFM pH 3.0 – 10 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona), MS- compatible phase

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Amino Acid
Analyzing Compounds Lysine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
D-Lysine
Lysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.