Amino Acids

Amino Acids

Synonyms

    Applications:

    HPLC Separation of Mixture of Non-Essential Amino Acids

    2019-03-11

     

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 20/80%
    Buffer H3PO4 – 0.1%
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds L-Aspartic Acid (Asp/D), L-Serine (Ser/S), L-Glutamic Acid Glu/E), L-Alanine Ala/A)

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Aspartic Acid
    D-Alanine
    DL-Alanine
    GLU (L-Glutamic acid)
    Glutamic Acid
    Serine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column

    2019-03-11

     

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 35/65%
    Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R)

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Arginine
    Asparagine
    Cysteine
    D-Isoleucine
    D-Leucine
    D-Valine
    DL-Isoleucine
    Histidine
    Isoleucine
    L-Cysteine
    L-Methionine
    L-Threonine
    Leucine
    Methionine
    Phenylalanine
    Proline
    Tryptophan
    Valine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Mixture of Essential Amino Acids on Primesep 100 Column

    2019-03-11

     

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 25/75%
    Buffer Gradient H2SO4 0.05-0.2% 25 min
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), L- Tryptophan (Trp/W)

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    D-Isoleucine
    D-Leucine
    D-Valine
    DL-Isoleucine
    Histidine
    Isoleucine
    L-Histidine hydrochloride monohydrate
    L-Isoleucine
    L-Methionine
    L-Threonine
    Methionine
    Phenylalanine
    Tryptophan
    Valine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column

    2019-03-11

     

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 35/65%
    Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Glutamin (Gln/Q), L-Glycine (Gly/G), L-Cysteine (Cys/C), L-Proline Pro/p), L-Tyrosine (Tyr/Y), L-Arginine (Arg/R)

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Arginine
    Cysteine
    Glycine
    L-Cysteine
    L-Glutamine
    Proline
    Tyrosine
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

    2009-07-16

    Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

    Condition

    Column Primesep D, 3.2×50 mm, 5 µm, 100A
    Mobile Phase IPA
    Buffer Formic Acid
    Flow Rate 0.5 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Glycine, Histidine, Sucrose, Polysorbate 20

    Application Column

    Primesep D

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Glycine
    Histidine
    Polysorbate
    Polysorbate 20
    Polysorbate 80
    Sucrose
    Tween 20
    Tween 80

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Amino Acids in Supplements Composition in Mixed-Mode

    2009-07-16

    Amino acids are essential components of numerous formulation. Health supplements can contain various amino acids and vitamins and require quantitation of each ingredients. Amino acids are very polar compounds with limited or no retention in reversed-phase chromatography. The most common approaches are reversed-phase chromatography with ion-pairing reagent and hydrophilic interaction chromatography (HILIC). Underivatized amino acids can be retained by combination of reversed-phase and cation exchange mechanism on Primesep 100 mixed-mode. Retention time is controlled by amount of acetonitrile, buffer and buffer pH. Method does not require ion-pairing reagent. This method is for UV detection. LC/MS, ELSD or Corona CAD can be employed for analysis of amino acids with trifluoroacteic acid or ammonium formate in the mobile phase. This approach can be used for HPLC analysis of all underivatized amino acids.

    Condition

    Column Primesep 100, 3.2×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 15/85%
    Buffer H3PO4
    Flow Rate 1.0 ml/min
    Detection UV, 200 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Theanine, Melatonin, GABA, 5- Hydroxytryptophan

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    5-Hydroxytryptophan
    Amino Acids
    Melatonin
    Theanine
    gamma-Aminobutyric Acid (GABA)

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography

    2009-07-16

    Dimethyl sulfoxide is important polar aprotic solvent, which is frequently used in pharmaceutical drug manufacturing, desolution, etc. DMSO is used as one of the solvents on protein chemistry due to universal ability to dissolve small molecules like amino acids. Amino acids and DMSO are very polar and have no retention on reversed-phase columns. In this HPLC application DMSO and glycine are separated on Primesep 100 mixed-mode column. DMSO is retained by weak reversed-phase mechanism and very low organic concentration is required in order to achieve any retention. Glycine, like any other underivatized amino acid, is retained by weak reversed-phase and moderate cation-exchange mechanism. Method uses acetonitrile/water/TFA gradient and UV detection.

    Condition

    Column Primesep 100, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer TFA
    Flow Rate 1.0 ml/min
    Detection UV, 210 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds DMSO, Glycine

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    DMSO (Dimethyl sulfoxide)
    Glycine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds

    2006-10-14

    Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection.

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer TFA – 0.2
    Flow Rate 1.0 ml/min
    Detection UV, 210 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Tyrosine, phenylalanine, Benzoic acid, mandelic acid, Benzylamine, Pyridine, Benzonitrile, Toluene

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Amino Acids
    Benzoic Acid
    Benzonitrile
    Benzylamine
    Mandelic Acid
    Organic Acids
    Phenylalanine
    Pyridine
    Toluene
    Tyrosine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Amino Acids Analysis in Acid Gradient Condition

    2006-09-18

    11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

    Condition

    Column Primesep 100, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 30/70%
    Buffer TFA , gradient  0.05-0.3 % , 25 min
    Flow Rate 1.0 ml/min
    Detection ELSD

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Aspartic acid, Glutamic acid, Alanine, Valine, Methionine, Isoleucine, Cysteine, Phenylalanine, Histidine, Lysine, Arginine

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Alanine
    Amino Acids
    Arginine
    Aspartic Acid
    Cysteine
    Glutamic Acid
    Histidine
    Isoleucine
    Lysine
    Methionine
    Phenylalanine
    Valine

    Application Detection:
    ELSD Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

    2005-01-30


    Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

    Condition

    Column Primesep 200, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN
    Buffer No
    Flow Rate 1.0 ml/min
    Detection UV, 195 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Aspartic acid,  Alanine, Valine, Methionine, Leucine

     

    Application Column

    Primesep 200

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    Alanine
    Amino Acids
    Aspartic Acid
    Leucine
    Methionine
    Valine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Hydrophobic and Hydrophilic Compound Separation

    2003-09-25

    Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

    Condition

    Column Primesep 100, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer H2SO4
    Flow Rate 1.0 ml/min
    Detection UV 210 nm

     

    Description

    Class of Compounds
    Drug, Acid, Hydrophilic, Ionizable, Nonpolar, Polar, Supplements
    Analyzing Compounds Cysteine,  L-cystine, 2,2-dimethylcystine,  2-methylcysteine, Benzoic Acid,  Toluene

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

    Select options
    Application Analytes:
    2,2-Dimethylcysteine
    2-Methylcysteine
    Amino Acids
    Benzoic Acid
    Cysteine
    L-Cystine
    Toluene

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.