D-Alanine

D-Alanine structural formula

CAS Number338-69-2
Molecular FormulaC3H7NO2
Molecular Weight89.094
InChI KeyQNAYBMKLOCPYGJ-UWTATZPHSA-N
LogP-1.86
Synonyms
  • D-Alanine
  • 338-69-2
  • 2: PN: RU2506269 PAGE: 13 claimed sequence
  • Ba 2776
  • (R)-Alanine
  • ALANINE, D-
  • D-(-)-Alanine
  • D(-)-?-Alanine
  • D-Alanin
  • D-alanina
  • D-?-Alanine
  • NSC 158286
  • EINECS 206-418-1
  • (R)-2-Aminopropionsaeure
  • D-alpha-Aminopropionsaeure
  • UNII-E3UDS4613U
  • (2R)-2-aminopropanoic acid
  • (R)-2-aminopropanoic acid
  • D(-)-a -Alanine
  • D(-)-alpha-Alanine
  • D-Ala
  • D-alpha-alanine
  • D-alpha-aminopropionic acid
  • DAL
  • delta-(-)-Alanine
  • delta-2-Aminopropionic acid
  • delta-Alanine
  • delta-alpha-Alanine
  • D-alanine zwitterion

Applications:

New HPLC Amino Acids Separation Compatible With Carbon Dating Technique

February 11, 2020

 

 

Hydroxyproline seems to be the most promising amino acid used in carbon dating when isolated from bone collagen. Separation of amino acids is challenging, especially without the use of ions or inorganic buffers that can interfere with Mass spectrometer (MS) or contaminate the sample with modern carbon. Amino acids are also not retained in reverse-phase chromatography. The ideal solution would be using water only to separate the amino acids. This would allow a direct coupling to MS. We were able to separate hydroxyproline from proline and other simple amino acids like glycine and alanine in HPLC on Newcrom AH column using water only as a mobile phase. Using water also allowed UV detection at 205 nm which can’t be done if using a buffer based on acetic or formic acid.
See more information on radiocarbon dating here.
The same method can be modified to get symmetrical peaks and higher efficiency if a mobile phase with ionic modifier such as formic acid is used.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Flow Rate 1.0 ml/min
Detection UV, 205 nm, CAD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Alanine, Glycine, Proline, Hydroxyproline

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Alanine
D-Alanine
Glycine
Hydroxyproline
Proline
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Non-Essential Amino Acids

March 11, 2019

 

Condition

Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Aspartic Acid (Asp/D), L-Serine (Ser/S), L-Glutamic Acid Glu/E), L-Alanine Ala/A)

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amino Acids
Aspartic Acid
D-Alanine
DL-Alanine
GLU (L-Glutamic acid)
Glutamic Acid
Serine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of D-Alanine on Newcrom R1 HPLC column

February 16, 2018
Separation of D-Alanine on Newcrom C18 HPLC column

D-Alanine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
D-Alanine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.