Tween 80

CAS Number9050-57-1
Molecular FormulaC32H60O10
Molecular Weight604.822
  • Polysorbate 80
  • Polysorbates
  • PSML
  • Sorbitan Derivatives
  • Sorbitan Monolaurate, Polyoxyethylene


HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80

chr_288.gif Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.  


Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds  Acid, Ion,  Hydrophobic, Ionizable
Analyzing Compounds Barium ion, Citric acid,  Sucrose, Tween 80

Application Analytes:
Citric Acid
Tween 80

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.


Column Primesep D, 3.2x50 mm, 5 µm, 100A
Mobile Phase IPA
Buffer Formic Acid
Flow Rate 0.5 ml/min
Detection ELSD


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Glycine, Histidine, Sucrose, Polysorbate 20

Application Analytes:
Amino Acids
Polysorbate 20
Polysorbate 80
Tween 20
Tween 80

Application Detection:
ELSD/MS Detection