Polysorbate

Synonyms

    Applications:

    HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

    2009-07-16
    Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

    Condition

    Column Primesep D, 3.2x50 mm, 5 µm, 100A
    Mobile Phase IPA
    Buffer Formic Acid
    Flow Rate 0.5 ml/min
    Detection ELSD
     

    Description

    Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
    Analyzing Compounds Glycine, Histidine, Sucrose, Polysorbate 20

    Application Analytes:
    Amino Acids
    Glycine
    Histidine
    Polysorbate
    Polysorbate 20
    Polysorbate 80
    Sucrose
    Tween 20
    Tween 80

    Application Detection:
    ELSD/MS Detection

    HPLC Retention of Polysorbate on Primesep D Column

    2008-08-22



    Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers. All oligomers are separated on Primesep D HPLC column. One of the oligomers can be used in quantitating polysorbate in various drug and food formulations.


    Application Analytes:
    Polysorbate
    Polysorbate 20
    Polysorbate 80

    Application Detection:
    ELSD/MS Detection