Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)


Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 195 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Aspartic acid,  Alanine, Valine, Methionine, Leucine
 
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

Application Detection:
UV Detection