DL-Isoleucine

DL-Isoleucine structural formula

CAS Number443-79-8
Molecular FormulaC6H13NO2
Molecular Weight131.176
InChI KeyAGPKZVBTJJNPAG-UHFFFAOYSA-N
LogP-0.720
Synonyms
  • DL-Isoleucine
  • Isoleucine
  • 443-79-8
  • 2-Amino-3-methylpentanoic acid
  • EINECS 207-139-8
  • FEMA No. 3295
  • NSC 9958
  • UNII-5HX0BYT4E3
  • 1160211-67-5
  • 198412-80-5
  • 19883-67-1
  • 205879-85-2
  • 224311-99-3
  • 228416-77-1
  • 676371-21-4

Applications:

HPLC Separation of Allo-Isoleucine, Isoleucine, Leucine on Primesep 100 Column

November 12, 2019


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HPLC Separation of Allo-Isoleucine  Isoleucine, Leucine on Primesep 100 Column Chr_1197

Condition

Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmFM pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Allo-Isoleucine, Isoleucine, Leucine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
D-Isoleucine
D-Leucine
DL-Isoleucine
Isoleucine
L-Alloisoleucine
L-Isoleucine
Leucine
allo-D-isoleucine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column

March 11, 2019

 

Condition

Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R)

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
L-Threonine
Leucine
Methionine
Phenylalanine
Proline
Tryptophan
Valine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Essential Amino Acids on Primesep 100 Column

March 11, 2019

 

Condition

Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer Gradient H2SO4 0.05-0.2% 25 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), L- Tryptophan (Trp/W)

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
L-Threonine
Methionine
Phenylalanine
Tryptophan
Valine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of DL-Isoleucine on Newcrom R1 HPLC column

February 16, 2018
Separation of DL-Isoleucine on Newcrom C18 HPLC column

DL-Isoleucine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
DL-Isoleucine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.