L-Alloisoleucine

L-Alloisoleucine structural formula

CAS Number1509-34-8
Molecular FormulaC6H13NO2
Molecular Weight131.176
InChI KeyAGPKZVBTJJNPAG-UHNVWZDZSA-N
LogP-0.720
Synonyms
  • L-Alloisoleucine
  • 1509-34-8
  • Alloisoleucine
  • allo-L-Isoleucine
  • BRN 1721791
  • EINECS 216-142-3
  • Isoleucine, allo-
  • L-Isoleucine, allo-
  • L-Isoleucine, 3-methyl-, threo-
  • Isoleucine, threo-
  • L-Isoleucine, threo-
  • L-Norvaline, 3-methyl-, threo-
  • Norvaline, 3-methyl-, threo-
  • NSC 206282
  • Pentanoic acid, 2-amino-3-methyl-, (S-(R*,S*))-
  • (2S,3R)-2-amino-3-methylpentanoate
  • (2S,3R)-2-amino-3-methylpentanoic acid
  • (3R)-LS-Isoleucine
  • 2-Amino-3-methyl-[S-(R*,S*)]-Pentanoate
  • 2-Amino-3-methyl-[S-(R*,S*)]-Pentanoic acid
  • Allo-Isoleucine
  • L(+)-Alloisoleucine
  • L-Allo-Isoleucine
  • [S-(R*,S*)]-2-Amino-3-methylpentanoate
  • [S-(R*,S*)]-2-Amino-3-methylpentanoic acid
  • alle
  • threo-3-methyl-L-Norvaline
  • threo-L-Isoleucine
  • L-alloisoleucine zwitterion

Applications:

HPLC Separation of Allo-Isoleucine, Isoleucine, Leucine on Primesep 100 Column

November 12, 2019

HPLC Method for D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine on Primesep 100 by SIELC Technologies

HPLC Separation of Allo-Isoleucine  Isoleucine, Leucine on Primesep 100 Column Chr_1197

High Performance Liquid Chromatography (HPLC) Method for Analysis of D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine 

Alloisoleucine is an amino acid with C6H13NO2 chemical formula. It is a key indicator when diagnosing Maple Syrup Urine Disease, when a body cannot metabolize amino acids in specific foods. Outside of that, it is also used in peptide synthesis and drug development.

Isoleucine is an amino acid with chemical formula C6H13NO2. It is a building block for proteins and is used in a variety of bodily functions. It helps form hemoglobin, regulating blood sugar, plays a key role in energy production, and increases the speed of muscle healing and growth. The easiest way to prevent a deficiency of it is through eating foods rich in protein, as those typically contain necessary amino acids.

Leucine is an amino acid with chemical formula C6H13NO2. It is key for protein synthesis, metabolism, tissue repair, and certain tasks that are also done by Isoleucine. It is known to activate mTOR cell signalng pathways, which stimulate protein synthesis and prevent breakdown of protein. It is found bin animal protein, plant-based protein, and nuts.

D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using CAD.

Condition

Column Primesep 100, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 5/95%
Buffer AmFM pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
D-Isoleucine
D-Leucine
DL-Isoleucine
Isoleucine
L-Alloisoleucine
L-Isoleucine
Leucine
allo-D-isoleucine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of L-Alloisoleucine on Newcrom R1 HPLC column

February 16, 2018
Separation of L-Alloisoleucine on Newcrom C18 HPLC column

L-Alloisoleucine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
L-Alloisoleucine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.