L-Alloisoleucine structural formula

CAS Number1509-34-8
Molecular FormulaC6H13NO2
Molecular Weight131.176
  • L-Alloisoleucine
  • 1509-34-8
  • Alloisoleucine
  • allo-L-Isoleucine
  • BRN 1721791
  • EINECS 216-142-3
  • Isoleucine, allo-
  • L-Isoleucine, allo-
  • L-Isoleucine, 3-methyl-, threo-
  • Isoleucine, threo-
  • L-Isoleucine, threo-
  • L-Norvaline, 3-methyl-, threo-
  • Norvaline, 3-methyl-, threo-
  • NSC 206282
  • Pentanoic acid, 2-amino-3-methyl-, (S-(R*,S*))-
  • (2S,3R)-2-amino-3-methylpentanoate
  • (2S,3R)-2-amino-3-methylpentanoic acid
  • (3R)-LS-Isoleucine
  • 2-Amino-3-methyl-[S-(R*,S*)]-Pentanoate
  • 2-Amino-3-methyl-[S-(R*,S*)]-Pentanoic acid
  • Allo-Isoleucine
  • L(+)-Alloisoleucine
  • L-Allo-Isoleucine
  • [S-(R*,S*)]-2-Amino-3-methylpentanoate
  • [S-(R*,S*)]-2-Amino-3-methylpentanoic acid
  • alle
  • threo-3-methyl-L-Norvaline
  • threo-L-Isoleucine
  • L-alloisoleucine zwitterion


HPLC Separation of Allo-Isoleucine, Isoleucine, Leucine on Primesep 100 Column

November 12, 2019

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HPLC Separation of Allo-Isoleucine  Isoleucine, Leucine on Primesep 100 Column Chr_1197


Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmFM pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase



Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Allo-Isoleucine, Isoleucine, Leucine


Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of L-Alloisoleucine on Newcrom R1 HPLC column

February 16, 2018
Separation of L-Alloisoleucine on Newcrom C18 HPLC column

L-Alloisoleucine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.