HPLC Method for D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine 

Alloisoleucine is an amino acid with C₆H₁₃NO₂ chemical formula. It is a key indicator when diagnosing Maple Syrup Urine Disease, when a body cannot metabolize amino acids in specific foods. Outside of that, it is also used in peptide synthesis and drug development.

Isoleucine is an amino acid with chemical formula C₆H₁₃NO₂. It is a building block for proteins and is used in a variety of bodily functions. It helps form hemoglobin, regulating blood sugar, plays a key role in energy production, and increases the speed of muscle healing and growth. The easiest way to prevent a deficiency of it is through eating foods rich in protein, as those typically contain necessary amino acids.

Leucine is an amino acid with chemical formula C₆H₁₃NO₂. It is key for protein synthesis, metabolism, tissue repair, and certain tasks that are also done by Isoleucine. It is known to activate mTOR cell signalng pathways, which stimulate protein synthesis and prevent breakdown of protein. It is found bin animal protein, plant-based protein, and nuts.

D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using CAD.

HPLC Method for Asparagine, L-Cysteine, Cysteine, Proline, Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, D-Leucine, Phenylalanine, Tryptophan, Histidine, Arginine, Amino Acids, Leucine, L-Threonine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Asparagine, L-Cysteine, Cysteine, Proline, Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, D-Leucine, Phenylalanine, Tryptophan, Histidine, Arginine, Amino Acids, Leucine, L-Threonine.

Amino acids are the building blocks of proteins. Based on their dietary requirement, they are classified into essential and non-essential amino acids. Essential amino acids cannot be synthesized by the human body in sufficient quantities and must be obtained from the diet. Non-essential amino acids, on the other hand, can be synthesized by the body and are not dependent on dietary intake.

It’s worth noting that while these amino acids are considered “non-essential” for adults under normal circumstances because the body can synthesize them, there are situations where some may become “conditionally essential.” This means that under certain conditions like illness, stress, or trauma, the body might not produce them in sufficient quantities, and dietary intake becomes necessary. Arginine, for instance, is considered conditionally essential, especially during periods of rapid growth, illness, or trauma.

Amino acids can be retained, separeted and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) as a buffer. This analysis method can be detected in the UV regime at 200 nm.

HPLC Method for Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine.

L-Threonine is an essential amino acid with the chemical formula C₄H₉NO₃.  It cannot be produced within the body and must be obtained through consuming it. It’s found in many protein-rich foods, including but not limited to eggs, meat, dairy, legumes, and seeds. It is necessary in the body as a building block of protein like collagen and elastin. The two proteins are crucial for skin, hair, and connective issue.

L-Valine is an essential amino acid with the chemical formula C₅H₁₁NO₂. It cannot be produced within the body and must be obtained through consuming it. It’s found in foods including but not limited to nuts, legumes, whole grains, and seeds. It is especially beneficial for athletes.  It is important for muscle repair,  growth, and energy regulation.

DL-Methionine is an essential amino acid with the chemical formula C₅H₁₁NO₂S.  It cannot be produced within the body and must be obtained through consuming it. It is required for protein synthesis. It also helps build and repair tissue including, but not limited to, skin, hair, muscles, and nails. In a veterinary context, DL-Methionine is used to address bladder issues in dogs.

L-Isoleucine is an essential amino acid with the chemical formula C₆H₁₃NO₂.  It cannot be produced within the body and must be obtained through consuming it. It is a building block of protein that are essential for muscle growth, repair, and other bodily functions.  It also helps regulate blood sugar levels and supports the immune system. It is found in foods like meat, fish, eggs, dairy, beans, lentils, nuts, and seeds.

L-Leucine is an essential amino acid with the chemical formula C₆H₁₃NO₂. It cannot be produced within the body and must be obtained through consuming it. It stimulates production of protein that are essential for muscle building and repair. Meats are the easiest way to get L-Leucine in significant amounts.

L-Phenylalanine is an essential amino acid with the chemical formula C₆H₉NO₂. It cannot be produced within the body and must be obtained through consuming it. It is typically found in high protein foods such as meat, eggs, and fish. Outside of being important for creation of protein, it is also used in treatment for skin disorders and depression.

L-Histidine is an essential amino acid with the chemical formula C₉H₁₁N₃O₂. It cannot be produced within the body and must be obtained through consuming it. It s fundamental for repair of damaged tissue, growth of muscles, and making of blood cells. Outside of protein, it also has the unique property of being able to act as a buffer to help maintain stable pH levels in the body. Sources of it include meat, fish, dairy products, beans, and nuts.

L-Tryptophan is an essential amino acid with the chemical formula C₁₁H₁₂N₂O₂. It cannot be produced within the body and must be obtained through consuming it. Like the other essential proteins, it is a building block for protein and muscle tissue, but it is also converted in the body into serotonin, which affects mood. L-Tryptophan is also used in treatments for severe PMS symptoms, depression, and insomnia. It is naturally found in red meat, poultry eggs, and dairy.

Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.