L-Cysteine

L-Cysteine structural formula

CAS Number52-90-4
Molecular FormulaC3H7NO2S
Molecular Weight121.151
InChI KeyXUJNEKJLAYXESH-REOHCLBHSA-N
LogP-2.49
Synonyms
  • L-Cysteine
  • 52-90-4
  • (R)-2-Amino-3-mercaptopropanoic acid
  • (R)-Cysteine
  • 2-Amino-3-mercaptopropionic acid
  • Cystein
  • Cysteine
  • Cysteine, L-
  • CYSTEINE, L-(+)-
  • Half-cystine
  • L-(+)-Cysteine
  • L-Alanine, 3-mercapto-
  • L-cisteina
  • L-Cystein
  • NSC 8746
  • Propanoic acid, 2-amino-3-mercapto-, (R)-
  • Thioserine
  • α-Amino-β-thiolpropionic acid
  • β-Mercaptoalanine
  • alpha-Amino-beta-mercaptopropanoic acid, L-
  • 2-Amino-3-mercaptopropanoic acid, (R)-
  • alpha-Amino-beta-mercaptopropionic acid, L-
  • (R)-2-Amino-3-mercaptopropionic acid
  • alpha-Amino-beta-thiolpropionic acid, L-
  • EINECS 200-158-2
  • FEMA No. 3263
  • Half cystine
  • beta-Mercaptoalanine, L-
  • NSC-8746
  • UNII-K848JZ4886
  • (+)-2-Amino-3-mercaptopropionic acid
  • (2R)-2-amino-3-mercapto-propanoic acid
  • (2R)-2-amino-3-mercaptopropanoate
  • (2R)-2-amino-3-mercaptopropanoic acid
  • (2R)-2-amino-3-sulfanyl-propanoic acid
  • (2R)-2-amino-3-sulfanylpropanoate
  • (2R)-2-amino-3-sulfanylpropanoic acid
  • (R)-(+)-cysteine
  • (R)-2-Amino-3-mercaptopropanoate
  • (R)-2-amino-3-mercapto-Propanoate
  • (R)-2-amino-3-mercapto-Propanoic acid
  • (R)-2-amino-3-mercaptopropanoic acid
  • 2-Amino-3-mercaptopropanoate
  • 2-Amino-3-mercaptopropanoic acid
  • 2-Amino-3-mercaptopropionate
  • 3-Mercapto-L-Alanine
  • Acetylcysteine
  • B-Mercaptoalanine
  • Carbocysteine
  • Cisteina
  • Cisteinum
  • Cysteinum
  • E 920
  • E-920
  • E920
  • FREE CYSTEINE
  • L Cysteine
  • L-2-Amino-3-mercaptopropanoate
  • L-2-Amino-3-mercaptopropanoic acid
  • L-Zystein
  • Polycysteine
  • alpha-Amino-beta-thiolpropionic acid
  • beta-Mercaptoalanine
  • (2R)-2-ammonio-3-mercaptopropanoate
  • L-cysteine zwitterion
  • 154605-72-8
  • 4371-52-2

Applications:

HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column

2022-03-10

Separation type: Liquid Chromatography Mixed-mode High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine and Cystine Cysteine is an amino acid that is an essential building block of a wide variety of proteins made and used throughout the entire body. Cystine is an oxidized dimer of cysteine that the body uses for redox reactions and as a linkage for proteins to keep their 3D structures. Cysteine and Cystine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Perchloric acid (HClO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.  

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Amino Acid
Analyzing Compounds Cysteine,  Cystine
 
Application Analytes:
Cysteine
Cystine
L-Cysteine

HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column

2019-03-11
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R)
 
Application Analytes:
Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
L-Threonine
Leucine
Methionine
Phenylalanine
Proline
Tryptophan
Valine

HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column

2019-03-11
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Glutamin (Gln/Q), L-Glycine (Gly/G), L-Cysteine (Cys/C), L-Proline Pro/p), L-Tyrosine (Tyr/Y), L-Arginine (Arg/R)
 
Application Analytes:
Amino Acids
Arginine
Cysteine
Glycine
L-Cysteine
L-Glutamine
Proline
Tyrosine

HPLC Application for Simultaneous Separation of Amino Acids, Hydrophilic Acidic and Hydrophobic Neutral Compounds

2007-12-06
Mixed-mode chromatography allows separating, in single run, compounds with vastly different properties. A method for separation of amino acids (cysteine, methylcysteine, cystine and dimethylcysteine) in the presence of carboxylic acid (benzoic) and hydrophobic neutral compounds was developed on Primesep 100 mixed-mode column. At lower pH ionization of carboxylic acids is suppressed. Amino acids are retained as basic compound based on reverse phase and cation exchange mechanisms. Carboxylic acids are retained on this column based on weak reverse phase mechanisms. Neutral compounds are retained by reverse phase mechanism as on any other column. Retention time of basic, zwitter-ionic and hydrophobic compound can be adjusted by manipulation of mobile phase composition. ELSD, UV or LC/MS detection can be used based on the properties of analytes and mobile phase selection.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Cysteine, Methylcysteine, Cystine, Dimethylcysteine,  Benzoic acid, Toluene,

Application Analytes:
2,2-Dimethylcysteine
2-Methylcysteine
Benzoic Acid
Cystine
L-Cysteine
Toluene

Application Detection:
UV Detection