CAS Number56-89-3
Molecular FormulaC6H12N2O4S2
Molecular Weight240.290
  • L-Cystine
  • 56-89-3
  • 4-04-00-03155
  • (-)-Cystine
  • [R-(R*,R*)]-3,3'-Dithiobis[2-aminopropanoic acid]
  • 3,3'-Dithiobis (2-aminopropionic acid)
  • 3,3'-Dithiobis(2-aminopropanoic acid)
  • Alanine, 3,3'-dithiobis-
  • Bis(β-amino-β-carboxyethyl) disulfide
  • cistina
  • Cystine
  • Cystine acid
  • Dicysteine
  • L-(-)-CYSTEINE
  • L-(-)-Cystine
  • L-Alanine, 3,3'-dithiobis-
  • L-Cysteine disulfide
  • L-Cystin
  • NSC 13203
  • Oxidized L-cysteine
  • Propanoic acid, 3,3'-dithiobis[2-amino-, [R-(R*,R*)]-
  • β,β'-Diamino-β,β'-dicarboxydiethyl disulfide
  • β,β'-Dithiodialanine
  • Alanine, 3,3'-dithiodi-
  • Bis(beta-amino-beta-carboxyethyl)disulfide
  • BRN 1728094
  • Cystine (L)-
  • beta,beta'-Diamino-beta,beta'-dicarboxydiethyldisulfide
  • beta,beta'-Dithioalanine, L-
  • (R-(R*,R*))-3,3'-Dithiobis(2-aminopropanoic acid)
  • 3,3'-Dithiobis(2-aminopropanoic acid), (R-(R*,R*))-
  • EINECS 200-296-3
  • Propanoic acid, 3,3'-dithiobis(2-amino-, (R-(R*,R*))-
  • (2R)-2-amino-3-[(2R)-2-amino-2-carboxy-ethyl]disulfanyl-propanoic acid
  • (2R)-2-amino-3-{[(2R)-2-amino-2-carboxyethyl]disulfanyl}propanoic acid
  • (R-(R*,R*))-3,3'-Dithiobis
  • (R-(R*,R*))-3,3'-Dithiobis(2-aminopropanoic acid)
  • 2-Amino-3-(2-amino-2-carboxy-ethyl)disulfanyl-propanoate
  • 2-Amino-3-(2-amino-2-carboxy-ethyl)disulfanyl-propanoic acid
  • 2-Amino-3-[(2-amino-2-carboxyethyl)dithio]propanoate
  • 2-Amino-3-[(2-amino-2-carboxyethyl)dithio]propanoic acid
  • 3,3'-Dithiobis
  • 3,3'-Dithiobis-L-alanine
  • 3,3'-Dithiobis[2-amino-[R-(R*,R*)]-Propanoate
  • 3,3'-Dithiobis[2-amino-[R-(R*,R*)]-Propanoic acid
  • 3,3'-Dithiodialanine
  • Bis(b-amino-b-carboxyethyl) disulfide
  • Bis(b-amino-beta-carboxyethyl) disulfide
  • Cysteine disulfide
  • Cystin
  • D(+)-3,3'-Dithiobis(2-aminopropanoate
  • D(+)-3,3'-Dithiobis(2-aminopropanoic acid
  • E921
  • Gelucystine
  • [R-(R*,R*)]-3,3'-Dithiobis
  • b,b'-Diamino-b,b'-dicarboxydiethyl disulfide
  • b,b'-Dithiodialanine
  • beta,beta'-Dithiobisalanine
  • beta,beta'-diamino-beta,beta'-dicarboxydiethyl disulfide
  • beta,beta'-dithiodialanine
  • bis(beta-amino-beta-carboxyethyl) disulfide
  • (2R,2'R)-3,3'-dithiobis(2-ammoniopropanoate)
  • L-cystine zwitterion
  • 154605-69-3
  • 24645-67-8


HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column


Separation type: Liquid Chromatography Mixed-mode High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine and Cystine Cysteine is an amino acid that is an essential building block of a wide variety of proteins made and used throughout the entire body. Cystine is an oxidized dimer of cysteine that the body uses for redox reactions and as a linkage for proteins to keep their 3D structures. Cysteine and Cystine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Perchloric acid (HClO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.  


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm


Class of Compounds Amino Acid
Analyzing Compounds Cysteine,  Cystine
Application Analytes:

HPLC Application for Simultaneous Separation of Amino Acids, Hydrophilic Acidic and Hydrophobic Neutral Compounds

Mixed-mode chromatography allows separating, in single run, compounds with vastly different properties. A method for separation of amino acids (cysteine, methylcysteine, cystine and dimethylcysteine) in the presence of carboxylic acid (benzoic) and hydrophobic neutral compounds was developed on Primesep 100 mixed-mode column. At lower pH ionization of carboxylic acids is suppressed. Amino acids are retained as basic compound based on reverse phase and cation exchange mechanisms. Carboxylic acids are retained on this column based on weak reverse phase mechanisms. Neutral compounds are retained by reverse phase mechanism as on any other column. Retention time of basic, zwitter-ionic and hydrophobic compound can be adjusted by manipulation of mobile phase composition. ELSD, UV or LC/MS detection can be used based on the properties of analytes and mobile phase selection.


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Cysteine, Methylcysteine, Cystine, Dimethylcysteine,  Benzoic acid, Toluene,

Application Analytes:
Benzoic Acid

Application Detection:
UV Detection