HPLC Method for Analysis of Cystine on Primesep 100 Column

HPLC Method for Cystine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Cystine

Cystine is an organic compound with the molecular formula C6H12N2O4S2.

Properties:
Appearance: Typically appears yellowish with a waxy, compact, and partially opaque texture.

Molecular weight: ~240.3 g/mol

Solubility: Soluble in water with HCL or NaOH.

Uses: Plays a crucial role in various biological processes due to its ability to stabilize protein structures and its role as a precursor to the antioxidant glutathione.

Cystine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 200 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm

Class of CompoundsDisulfide Amino Acid
Analyzing CompoundsCystine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cystine
Application Detection:
UV Detection
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