HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine and Cystine

Cysteine is an amino acid that is an essential building block of a wide variety of proteins made and used throughout the entire body. Cystine is an oxidized dimer of cysteine that the body uses for redox reactions and as a linkage for proteins to keep their 3D structures. Cysteine and Cystine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV, 200 nm
Class of Compounds
Amino Acid
Analyzing CompoundsCysteine,  Cystine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Cysteine
Cystine
L-Cysteine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.