HPLC Method for Cysteine, Cystine, L-Cysteine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine, Cystine, L-Cysteine.
L-Cysteine is an amino acid with the chemical formula C5H10N2O3. It is primarily a building block for protein, but it also has antioxidant effects. On occasion, it is used to support people dealing with cancer, diabetes, and hangover, but there is yet to be substantial evidence that it works. Poultry, egg, beef, and whole grains are rich sources of the amino acid. You can find detailed UV spectra of Cysteine and information about its various lambda maxima by visiting the following link.Cystine is an amino acid with the molecular formula C6H12N2O4S2. It plays a crucial role in various biological processes due to its ability to stabilize protein three-dimensional structures. It serves as a major precursor for the synthesis of glutathione. UV spectra of Cystine and information about its various lambda maxima by visiting the following link.Cysteine, Cystine, L-Cysteine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Cysteine, Cystine, L-Cysteine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Cystine
L-Cysteine



