CAS Number | 56-85-9 |
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Molecular Formula | C5H10N2O3 |
Molecular Weight | 146.146 |
InChI Key | ZDXPYRJPNDTMRX-VKHMYHEASA-N |
LogP | -3.64 |
Synonyms |
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Applications:
HPLC Separation of Amino Acids on Newcrom AH Column
October 1, 2020
HPLC Method for Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine on Newcrom AH by SIELC Technologies
Amino acids are the building blocks of proteins. Based on their dietary requirement, they are classified into essential and non-essential amino acids. Essential amino acids cannot be synthesized by the human body in sufficient quantities and must be obtained from the diet. Non-essential amino acids, on the other hand, can be synthesized by the body and are not dependent on dietary intake.
It’s worth noting that while these amino acids are considered “non-essential” for adults under normal circumstances because the body can synthesize them, there are situations where some may become “conditionally essential.” This means that under certain conditions like illness, stress, or trauma, the body might not produce them in sufficient quantities, and dietary intake becomes necessary. Arginine, for instance, is considered conditionally essential, especially during periods of rapid growth, illness, or trauma.
You can find detailed UV spectra of Aspartic Acid and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.
You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.
Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine can be retained and analyzed using the Newcrom AH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Formic Acid buffer. Detection is performed using CAD.
Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN – 50% |
Buffer | Formic Acid – 0.1% |
Flow Rate | 1.0 ml/min |
Detection | CAD |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
Analyzing Compounds | Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine |
Application Column
Newcrom AH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Glutamic Acid
Glycine
L-Glutamine
HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column
March 11, 2019
HPLC Method for L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids.
Glutamine is an amino acid with the chemical formula C5H10N2O3. It is a building block for protein, but it also supports the immune system, gut health, and detoxification. It is usually found in meat, fish, eggs, dairy, and whole grains; though some consider it the most abundant amino acid in the body. People also take glutamine as treatment for sickle cell disease, healing of burns, recovery after surgery, and injuries.
L-Glycine is an important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. It has he chemical formula C₂H₅NO₂. It is water-soluble and plays a critical role in protein synthesis, peptide modification, and metabolic studies. It can be found in meat., eggs. and bones.
L-Cysteine is an amino acid with the chemical formula C5H10N2O3. It is primarily a building block for protein, but it also has antioxidant effects. On occasion, ot os used to support people dealing with cancer, diabetes, and hangover, but there is yet to be substantial evidence that it works. Poultry, egg, beef, and whole grains are rich sources of the amino acid.
L-Proline is an amino acid with the chemical formula C5H9NO2. It is naturally produced in the body, but it is also recommended to be consumed through the diet. It can be found in meat, fish, and dairy. It is used for skin healing and treatment of other skin conditions as it is an essential component of collagen. It is important for upkeep of joints and tendons, as well as heart muscles.
L-Tyrosine is an amino acid with the chemical formula C9H11NO3. It is naturally used in the body to create neurotransmitters like dopamine and norepinephrine. While it is produced in the body naturally, it can also be consumed in beef, pork, fish, dairy products, and beans. As a supplement, it is also used to treat Phenylketonuria (PKU).
L-Arginine is an amino acid with the chemical formula C9H11NO3. It is a precursor for nitric acid, making it crucial for blood flow and general cardiovascular health. It can help lower blood pressure, help with chest pain, and improve symptoms of Peripheral arterial disease (PAD).
L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Column | Primesep 100, 4.6 x 250 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 35/65% |
Buffer | H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min |
Flow Rate | 1.0 ml/min |
Detection | UV, 200 nm |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
Analyzing Compounds | L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Arginine
Cysteine
Glycine
L-Cysteine
L-Glutamine
Proline
Tyrosine
Separation of L-Glutamine on Newcrom R1 HPLC column
February 16, 2018
L-Glutamine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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