L-Glutamine

L-Glutamine structural formula

CAS Number56-85-9
Molecular FormulaC5H10N2O3
Molecular Weight146.146
InChI KeyZDXPYRJPNDTMRX-VKHMYHEASA-N
LogP-3.64
Synonyms
  • L-Glutamine
  • 56-85-9
  • Glutamine
  • 4-04-00-03038
  • (S)-2,5-Diamino-5-oxopentanoic acid
  • 2-AMINOGLUTARAMIC ACID
  • Acustasin
  • Cebrogen
  • Glavamin
  • Glutamax
  • Glutamic acid 5-amide
  • Glutamic acid amide
  • GLUTAMINE, L-
  • L-(+)-GLUTAMINE
  • L-2-Aminoglutaramidic acid
  • Levoglutamid
  • levoglutamida
  • Levoglutamide
  • L-Glutamic acid ?-amide
  • NSC 27421
  • Pentanoic acid, 2,5-diamino-5-oxo-, (S)-
  • Saforis
  • Stimulina
  • ?-Glutamine
  • 2-Aminoglutaramic acid, L-
  • BRN 1723797
  • 2,5-Diamino-5-oxopentanoic acid, (S)-
  • EINECS 200-292-1
  • FEMA No. 3684
  • Miglu-P
  • Nutrestore
  • UNII-0RH81L854J
  • (2S)-2,5-diamino-5-oxopentanoate
  • (2S)-2,5-diamino-5-oxopentanoic acid
  • (2S)-2-amino-4-carbamoylbutanoate
  • (2S)-2-amino-4-carbamoylbutanoic acid
  • (S)-2,5-Diamino-5-oxopentanoate
  • (S)-2,5-diamino-5-oxopentanoic acid
  • Glumin
  • L-2-aminoglutaramic acid
  • L-Glutamic acid 5-amide
  • L-Glutamid
  • L-Glutamide
  • L-Glutamin
  • L-Glutaminsaeure-5-amid
  • L-glutamic acid gamma-amide
  • Levoglutamidum
  • Levoglutamina
  • Polyglutamine
  • gamma-Glutamine
  • (2S)-5-amino-2-ammonio-5-oxopentanoate
  • L-glutamine zwitterion
  • 32640-56-5

Applications:

HPLC Separation of Amino Acids on Newcrom AH Column

October 1, 2020

HPLC Method for Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine on Newcrom AH by SIELC Technologies

Amino acids are the building blocks of proteins. Based on their dietary requirement, they are classified into essential and non-essential amino acids. Essential amino acids cannot be synthesized by the human body in sufficient quantities and must be obtained from the diet. Non-essential amino acids, on the other hand, can be synthesized by the body and are not dependent on dietary intake.

It’s worth noting that while these amino acids are considered “non-essential” for adults under normal circumstances because the body can synthesize them, there are situations where some may become “conditionally essential.” This means that under certain conditions like illness, stress, or trauma, the body might not produce them in sufficient quantities, and dietary intake becomes necessary. Arginine, for instance, is considered conditionally essential, especially during periods of rapid growth, illness, or trauma.

You can find detailed UV spectra of Aspartic Acid and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.

Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine can be retained and analyzed using the Newcrom AH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Formic Acid buffer. Detection is performed using CAD.

 

Condition

Column Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 50%
Buffer  Formic Acid – 0.1%
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine

 

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Aspartic Acid
Glutamic Acid
Glycine
L-Glutamine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column

March 11, 2019

HPLC Method for L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids.

Glutamine is an amino acid with the chemical formula C5H10N2O3. It is a building block for protein, but it also supports the immune system, gut health, and detoxification. It is usually found in meat, fish, eggs, dairy, and whole grains; though some consider it the most abundant amino acid in the body. People also take glutamine as treatment for sickle cell disease, healing of burns, recovery after surgery, and injuries.

L-Glycine is an important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. It has he chemical formula C₂H₅NO₂. It is water-soluble and plays a critical role in protein synthesis, peptide modification, and metabolic studies. It can be found in meat., eggs. and bones.

L-Cysteine is an amino acid with the chemical formula C5H10N2O3. It is primarily a building block for protein, but it also has antioxidant effects. On occasion, ot os used to support people dealing with cancer, diabetes, and hangover, but there is yet to be substantial evidence that it works. Poultry, egg, beef, and whole grains are rich sources of the amino acid.

L-Proline is an amino acid with the chemical formula C5H9NO2. It is naturally produced in the body, but it is also recommended to be consumed through the diet. It can be found in meat, fish, and dairy. It is used for skin healing and treatment of other skin conditions as it is an essential component of collagen. It is important for upkeep of joints and tendons, as well as heart muscles.

L-Tyrosine is an amino acid with the chemical formula C9H11NO3. It is naturally used in the body to create neurotransmitters like dopamine and norepinephrine. While it is produced in the body naturally, it can also be consumed in beef, pork, fish, dairy products, and beans. As a supplement, it is also used to treat Phenylketonuria (PKU).

L-Arginine is an amino acid with the chemical formula C9H11NO3. It is a precursor for nitric acid, making it crucial for blood flow and general cardiovascular health. It can help lower blood pressure, help with chest pain, and improve symptoms of Peripheral arterial disease (PAD).

L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition

Column Primesep 100, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Glutamine, Glycine, Cysteine, L-Cysteine, Proline, Tyrosine, Arginine, Amino Acids

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Amino Acids
Arginine
Cysteine
Glycine
L-Cysteine
L-Glutamine
Proline
Tyrosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of L-Glutamine on Newcrom R1 HPLC column

February 16, 2018
Separation of L-Glutamine on Newcrom C18 HPLC column

L-Glutamine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
L-Glutamine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.